Apr 10, 2020

A high-throughput screening platform for pigment regulating agents using pluripotent stem cell derived melanocytes.

BioRxiv : the Preprint Server for Biology
V. ParatJulien Maruotti


In this study, we describe a simple and straight-forward assay using induced pluripotent stem cell derived melanocytes and high-throughput flow cytometry, to screen and identify pigment regulating agents. The assays is based on the correlation between forward light-scatter characteristics and melanin content, with pigmented cells displaying high light absorption/low forward light-scatter, while the opposite is true for lowly pigmented melanocytes, as a result of genetic background or chemical treatments. Orthogonal validation is then performed by regular melanin quantification. Such approach was validated using a set of 80 small molecules, and yielded a confirmed hit. The assay described in this study may prove a useful tool to identify modulators of melanogenesis in human melanocytes.

  • References
  • Citations


  • We're still populating references for this paper, please check back later.
  • References
  • Citations


  • This paper may not have been cited yet.

Mentioned in this Paper

Environmental Infrastructure
Metaplastic Cell Transformation
Genome Sequencing
Biological Evolution
Physiological Processes

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Comptes rendus des séances de la Société de biologie et de ses filiales
M Boue-GrabotJ Cambar
Proceedings of the National Academy of Sciences of the United States of America
Xavier NissanChristine Baldeschi
© 2020 Meta ULC. All rights reserved