PMID: 11927686Apr 3, 2002Paper

Interactive effect of chondroitin sulphate C and hyaluronan on fluid movement across rabbit synovium

The Journal of Physiology
S SabaratnamJ R Levick

Abstract

The polysaccharide hyaluronan (HA) conserves synovial fluid by keeping outflow low and almost constant over a wide pressure range ('buffering'), but only at concentrations associated with polymer domain overlap. We therefore tested whether polymer interactions can cause buffering, using HA-chondroitin sulphate C (CSC) mixtures. Also, since it has been found that capillary filtration is insensitive to the Starling force interstitial osmotic pressure in frog mesenteries, this was assessed in synovium. Hyaluronan at non-buffering concentrations (0.50-0.75 mg ml(-1)) and/or 25 mg ml(-1) CSC (osmotic pressure 68 cmH(2)O) was infused into knees of anaesthetised rabbits in vivo. Viscometry and chromatography confirmed that HA interacts with CSC. Pressure (P(j)) versus trans-synovial flow (;Q(s)) relations were measured.;Q(s) was outwards for HA alone (1.2 +/- 0.9 microl min(-1) at 3 cmH(2)O, mean +/- S.E.M.; n = 6). CSC diffused into synovium and changed;Q(s) to filtration at low P(j) (-4.1 microl min(-1), 3 cmH(2)O, n = 5, P < 0.02, t test). Filtration ceased upon circulatory arrest (n = 3). At higher P(j), 0.75 mg ml(-1) HA plus CSC buffered;Q(s) to approximately 3 microl min(-1) over a wide range of P(j), with an outflow increase o...Continue Reading

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Citations

Sep 19, 2012·Lymphatic Research and Biology·Melissa A RobertsJeremy Goldman
Feb 16, 2013·European Journal of Orthopaedic Surgery & Traumatology : Orthopédie Traumatologie·Travis E CleggCyril Mauffrey
Apr 10, 2004·The Journal of Physiology·R H AdamsonF E Curry
Apr 13, 2005·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Peter J Coleman

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