Interactome analysis reveals that FAM161A, deficient in recessive retinitis pigmentosa, is a component of the Golgi-centrosomal network

Human Molecular Genetics
Silvio Alessandro Di GioiaCarlo Rivolta

Abstract

Defects in FAM161A, a protein of unknown function localized at the cilium of retinal photoreceptor cells, cause retinitis pigmentosa, a form of hereditary blindness. By using different fragments of this protein as baits to screen cDNA libraries of human and bovine retinas, we defined a yeast two-hybrid-based FAM161A interactome, identifying 53 bona fide partners. In addition to statistically significant enrichment in ciliary proteins, as expected, this interactome revealed a substantial bias towards proteins from the Golgi apparatus, the centrosome and the microtubule network. Validation of interaction with key partners by co-immunoprecipitation and proximity ligation assay confirmed that FAM161A is a member of the recently recognized Golgi-centrosomal interactome, a network of proteins interconnecting Golgi maintenance, intracellular transport and centrosome organization. Notable FAM161A interactors included AKAP9, FIP3, GOLGA3, KIFC3, KLC2, PDE4DIP, NIN and TRIP11. Furthermore, analysis of FAM161A localization during the cell cycle revealed that this protein followed the centrosome during all stages of mitosis, likely reflecting a specific compartmentalization related to its role at the ciliary basal body during the G0 phase....Continue Reading

References

Jul 1, 1991·The Journal of Cell Biology·N HirokawaS T Brady
Jan 12, 2001·Molecular and Cellular Biology·Z YangL S Goldstein
Feb 13, 2001·Biochemical and Biophysical Research Communications·Y R HongS L Howng
Jul 14, 2001·Molecular Biology of the Cell·K Chabin-BrionC Poüs
Aug 10, 2001·The Journal of Biological Chemistry·C M HalesJ R Goldenring
May 17, 2002·Human Molecular Genetics·Carlo RivoltaThaddeus P Dryja
Jul 24, 2002·The Journal of Cell Biology·Ying XuNobutaka Hirokawa
Jan 10, 2003·Nucleic Acids Research·Brigitte BoeckmannMichel Schneider
Aug 2, 2003·Current Opinion in Cell Biology·Antonino ColanziVivek Malhotra
May 26, 2004·Biochemical and Biophysical Research Communications·Conor P HorganMary W McCaffrey
Jul 23, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Qin LiuEric A Pierce
Aug 5, 2006·Science·Veena Singla, Jeremy F Reiter
Nov 23, 2006·Lancet·Dyonne T HartongThaddeus P Dryja
Jan 24, 2007·Journal of Neuroscience Research·Rafal Butowt, Christopher S von Bartheld
Jun 5, 2007·Nature Genetics·Anneke I den HollanderRonald Roepman
Sep 20, 2007·Journal of Cell Science·Aurélie Hoppeler-LebelAnne-Marie Tassin
Oct 24, 2007·The Journal of Cell Biology·Susanne GraserErich A Nigg
Jul 2, 2008·Methods in Molecular Biology·Stef J F Letteboer, Ronald Roepman
Oct 18, 2008·Genome Biology·Martin KrallingerAlfonso Valencia
Dec 30, 2008·PLoS Genetics·John A FollitGregory J Pazour
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jun 6, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Sudipto Roy
Jun 11, 2009·Seminars in Cell & Developmental Biology·Irene Barinaga-Rementeria Ramirez, Martin Lowe
Mar 10, 2010·The Journal of Cell Biology·Christine Sütterlin, Antonino Colanzi
Mar 10, 2010·Nature Reviews. Genetics·Alan F WrightShomi S Bhattacharya
Mar 11, 2010·Biochemical and Biophysical Research Communications·Conor P HorganMary W McCaffrey
Apr 17, 2010·Nature Reviews. Genetics·Sarah C Goetz, Kathryn V Anderson
May 15, 2010·The Journal of Biological Chemistry·Zhe WangRobert Z Qi
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jing DuHusseini K Manji
Aug 14, 2010·American Journal of Human Genetics·Thomas LangmannCarlo Rivolta
Aug 14, 2010·American Journal of Human Genetics·Dikla Bandah-RozenfeldDror Sharon
Dec 17, 2010·Human Molecular Genetics·Christina F ChakarovaShomi S Bhattacharya
Dec 23, 2010·Ophthalmic Genetics·Jutta HoschKnut Stieger
Jan 7, 2011·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Aoife M Waters, Philip L Beales
Apr 22, 2011·The New England Journal of Medicine·Friedhelm HildebrandtNicholas Katsanis

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