Intercalation of Zn(II) and Cu(II) complexes of the cyclic polyamine Neotrien into DNA: equilibria and kinetics

Journal of Inorganic Biochemistry
Tarita BiverClaudia Giorgi

Abstract

The equilibria and kinetics of the interaction of the Zn(II) and Cu(II) complexes of the macrocyclic polyamine 2,5,8,11-tetraaza[12]-[12](2,9)[1,10]-phenanthrolinophane (Neotrien) with calf thymus DNA have been investigated at pH=7.0 and T=25 degrees C by spectrophotometry, spectrofluorimetry and stopped-flow method. At low dye/polymer ratios both complexes bind to DNA according to the excluded site model. At high dye/polymer ratios the binding displays cooperative features. The logarithm of the binding constant depends linearly on -log[NaCl]. The kinetic results suggest the D + S <==> D, S <==> DS mechanism where the metal complexes (D) react with the DNA sites (S) leading to fast formation of an externally bound form (D,S) which, in turn, is converted into internally bound complex (DS) by intercalation. The binding constants, evaluated as ratios of rate constants, agree with those obtained from equilibrium binding experiments, thus confirming the validity of the proposed model. Fluorescence titrations, where the metal-Neotrien complexes were added to DNA previously saturated with ethidium bromide (EB), show that both complexes displace EB from the DNA cavities. The reverse process, i.e. the addition of excess ethidium to the ...Continue Reading

References

May 9, 1987·Biophysical Chemistry·H DieblerM Venturini
Jul 28, 1968·Journal of Molecular Biology·W Müller, D M Crothers
May 10, 1983·Biochemistry·R F PasternackJ J Villafranca
Apr 27, 1993·Biochemistry·F J Meyer-Almes, D Porschke
Aug 22, 1996·Nature·D B HallJ K Barton
Mar 7, 1997·Science·P J DandlikerJ K Barton
Dec 26, 2001·Chemical Reviews·K E ErkkilaJ K Barton
Aug 13, 2002·Current Medicinal Chemistry·Adrienne Adams
Sep 19, 2003·Archives of Biochemistry and Biophysics·Tarita BiverMarcella Venturini

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Citations

Mar 27, 2007·Journal of Inorganic Biochemistry·Arturo SilvestriVincenzo Turco Liveri
Sep 15, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Carla BazzicalupiMarcella Venturini
Aug 19, 2014·Physical Chemistry Chemical Physics : PCCP·A BiancardiM Venturini
Aug 24, 2019·Chemistry : a European Journal·Dalila RoccoFabio Marchetti

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