PMID: 9535458Apr 16, 1998Paper

Intercellular adhesion molecule-1 regulation in the canine lung after cardiopulmonary bypass

The Journal of Thoracic and Cardiovascular Surgery
W J DreyerG L Kukielka

Abstract

Neutrophil sequestration in the lung after cardiopulmonary bypass has been shown to be dependent on the adhesion molecule CD18. Thus we sought to determine whether endothelial expression of intercellular adhesion molecule-1 (a ligand for CD18) in pulmonary capillaries mediates neutrophil adhesion in this setting. Seven adult mongrel dogs underwent 90 minutes of hypothermic cardiopulmonary bypass with 60 minutes of cardioplegic arrest. After warming, dogs were reperfused for up to 9 hours and lung biopsy specimens were obtained. Lung tissue was examined by Northern and Western blot analysis and by immunohistologic methods. Three sham-operated dogs served as time-matched controls. Northern blots demonstrated increased expression of intercellular adhesion molecule-1 messenger ribonucleic acid within 5 minutes of cessation of bypass (or approximately 30 minutes after aortic crossclamp release), which persisted at 9 hours of recovery and was not present in controls. Western blots showed intercellular adhesion molecule-1 protein expression before bypass but a measurable increase in intercellular adhesion molecule-1 protein in four of seven dogs in the bypass group by the ninth hour of recovery. Pulmonary neutrophil accumulation 9 hou...Continue Reading

References

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Citations

Nov 18, 2006·Journal of Thrombosis and Thrombolysis·Diana MühlJános Lantos
Sep 7, 2007·Journal of Cardiac Surgery·Shahzad G Raja, Geoffrey A Berg
Apr 12, 2002·Chest·Calvin S H NgAhmed A Arifi
Mar 27, 2003·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Sarah TabbuttEllis J Neufeld

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