Intercellular adhesion promotes clonal mixing in growing bacterial populations

Journal of the Royal Society, Interface
Anton KanJim Haseloff

Abstract

Dense bacterial communities, known as biofilms, can have functional spatial organization driven by self-organizing chemical and physical interactions between cells, and their environment. In this work, we investigated intercellular adhesion, a pervasive property of bacteria in biofilms, to identify effects on the internal structure of bacterial colonies. We expressed the self-recognizing ag43 adhesin protein in Escherichia coli to generate adhesion between cells, which caused aggregation in liquid culture and altered microcolony morphology on solid media. We combined the adhesive phenotype with an artificial colony patterning system based on plasmid segregation, which marked clonal lineage domains in colonies grown from single cells. Engineered E. coli were grown to colonies containing domains with varying adhesive properties, and investigated with microscopy, image processing and computational modelling techniques. We found that intercellular adhesion elongated the fractal-like boundary between cell lineages only when both domains within the colony were adhesive, by increasing the rotational motion during colony growth. Our work demonstrates that adhesive intercellular interactions can have significant effects on the spatial o...Continue Reading

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Citations

May 28, 2019·Nature Communications·Yusuf Ilker YamanAskin Kocabas
May 27, 2020·PLoS Biology·William P J SmithKevin R Foster
Jun 9, 2020·Biochemical Society Transactions·Jamie A Davies, Fokion Glykofrydis
Oct 28, 2020·Nature Communications·William P J SmithKevin R Foster
Mar 23, 2021·The ISME Journal·QinQin YuOskar Hallatschek
Jun 4, 2020·ACS Synthetic Biology·Vasily KantslerMunehiro Asally

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Methods Mentioned

BETA
PCR

Software Mentioned

CellModeller
Ubuntu

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