Intercellular diffusion of a fluorescent sucrose analog via the septal junctions in a filamentous cyanobacterium

MBio
Dennis J NürnbergConrad W Mullineaux

Abstract

Many filamentous cyanobacteria produce specialized nitrogen-fixing cells called heterocysts, which are located at semiregular intervals along the filament with about 10 to 20 photosynthetic vegetative cells in between. Nitrogen fixation in these complex multicellular bacteria depends on metabolite exchange between the two cell types, with the heterocysts supplying combined-nitrogen compounds but dependent on the vegetative cells for photosynthetically produced carbon compounds. Here, we used a fluorescent tracer to probe intercellular metabolite exchange in the filamentous heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120. We show that esculin, a fluorescent sucrose analog, is incorporated by a sucrose import system into the cytoplasm of Anabaena cells. The cytoplasmic esculin is rapidly and reversibly exchanged across vegetative-vegetative and vegetative-heterocyst cell junctions. Our measurements reveal the kinetics of esculin exchange and also show that intercellular metabolic communication is lost in a significant fraction of older heterocysts. SepJ, FraC, and FraD are proteins located at the intercellular septa and are suggested to form structures analogous to gap junctions. We show that a ΔsepJ ΔfraC ΔfraD tr...Continue Reading

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Citations

Nov 8, 2015·Biochimica Et Biophysica Acta·Ann Magnuson, Tanai Cardona
Jan 11, 2016·Trends in Microbiology·Enrique FloresIris Maldener
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Amin Omairi-NasserRobert Haselkorn
Jan 4, 2017·MBio·Mercedes Nieves-MoriónEnrique Flores
Aug 2, 2018·Environmental Microbiology·Antonia Herrero, Enrique Flores
Jan 30, 2019·Life·Ann Magnuson
Aug 14, 2018·Inflammatory Bowel Diseases·Alexander Rodriguez-Palacios, Fabio Cominelli
May 17, 2019·Plant & Cell Physiology·Elvira Olmedo-VerdAlicia M Muro-Pastor
Feb 11, 2020·Molecular Microbiology·Benjamin L SpringsteinTal Dagan
Apr 14, 2020·Environmental Microbiology·Mercedes Nieves-MoriónRachel A Foster
Sep 21, 2017·Frontiers in Cellular and Infection Microbiology·Jan BornikoelIris Maldener
Feb 6, 2017·Proceedings of the National Academy of Sciences of the United States of America·Zhenggao ZhengJindong Zhao
Sep 2, 2017·FEMS Microbiology Letters·Laura A Antonaru, Dennis J Nürnberg
Feb 17, 2017·FEMS Microbiology Reviews·Antonia HerreroEnrique Flores
Mar 9, 2018·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Neelam DubeySuvendra Nath Bagchi
Dec 24, 2018·Life·Enrique FloresConrad W Mullineaux
Oct 29, 2020·FEBS Open Bio·Benjamin L SpringsteinKarina Stucken
Dec 19, 2020·Computational and Structural Biotechnology Journal·Keisuke InomuraMichael J Follows
Dec 23, 2020·Life·Benjamin L SpringsteinKarina Stucken
Mar 7, 2021·Current Opinion in Microbiology·Ann-Katrin Kieninger, Iris Maldener

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Methods Mentioned

BETA
electron microscopy
fluorescence recovery after photobleaching
Fluorescence
PCR
electrophoresis
light microscopy
fluorescence imaging
PCRs

Software Mentioned

ImageJ
Leica Application Suite Advanced
ChemAxon
Marvin

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