Intercellular transmission of Seneca Valley virus mediated by exosomes

Veterinary Research
Guowei XuXiangtao Liu

Abstract

Seneca Valley virus (SVV) is a non-encapsulated single-stranded positive-strand RNA virus whose transmission routes have not yet been fully elucidated. Exosomes have been implicated in the intercellular transport of a variety of materials, such as proteins, RNA, and liposomes. However, whether exosomes can mediate SVV intercellular transmission remains unknown. In this study, we extracted exosomes from SVV-infected IBRS-2 cells to investigate intercellular transmission. Our results suggest that the intercellular transmission of SVV is mediated by exosomes. The results of co-localization and RT-qPCR studies showed that exosomes harbor SVV and enable the virus to proliferate in both susceptible and non-susceptible cells. Furthermore, the replication of SVV was inhibited when IBRS-2 cells were treated with interfering RNA Rab27a and exosome inhibitor GW4869. Finally, neutralization experiments were performed to further verify whether the virus was encapsulated by the exosomes that mediated transmission between cells. It was found that exosome-mediated intercellular transmission was not blocked by SVV-specific neutralizing antibodies. This study reveals a new transmission route of SVV and provides clear evidence regarding the patho...Continue Reading

References

Jun 30, 2004·Trends in Microbiology·Annegret Pelchen-MatthewsMark Marsh
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rebecca D Wiley, Suryaram Gummuluru
Nov 1, 2007·Journal of the National Cancer Institute·P Seshidhar ReddyPaul L Hallenbeck
Dec 8, 2009·Nature Cell Biology·Matias OstrowskiClotilde Thery
Apr 21, 2010·The Journal of Cell Biology·Chieh HsuMikael Simons
Jun 17, 2010·Neuromolecular Medicine·Bill X WuYusuf A Hannun
Jun 5, 2012·Nature Cell Biology·Maria Francesca BaiettiGuido David
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Jul 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Vedashree RamakrishnaiahLuc J W van der Laan
Feb 12, 2014·Journal of Hepatology·François-Loïc Cosset, Marlène Dreux
Jun 7, 2015·Cellular and Molecular Life Sciences : CMLS·Didier VilettePascal Leblanc
May 24, 2016·Transboundary and Emerging Diseases·P CanningL Karriker
Jun 18, 2016·Emerging Infectious Diseases·Raquel A LemeAmauri A Alfieri
May 26, 2017·Transboundary and Emerging Diseases·Z ZhuH Zheng
Feb 1, 2018·Chemical Reviews·Huilin ShaoHakho Lee
Apr 22, 2018·Molecular Cancer·Zhenqiang SunWeitang Yuan
Jun 2, 2018·Journal of Lipid Research·Claudia VerderioPaola Giussani
Dec 18, 2018·International Journal of Cancer. Journal International Du Cancer·Dajiang GuoKimberley A Beaumont
Feb 8, 2019·Advances in Experimental Medicine and Biology·Hüseyin AbdikFikrettin Şahin
Jun 10, 2019·Veterinary Microbiology·Keshan ZhangHaixue Zheng

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Datasets Mentioned

BETA
KY74510

Methods Mentioned

BETA
electron microscopy
electrophoresis
PCR
transfection

Software Mentioned

NTA

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