PMID: 12761202May 23, 2003Paper

Interconversion of the product specificity of type I eubacterial farnesyl diphosphate synthase and geranylgeranyl diphosphate synthase through one amino acid substitution

Journal of Biochemistry
Takashi KawasakiTohru Dairi

Abstract

Prenyltransferases catalyze the sequential condensation of isopentenyl diphosphate into prenyl diphosphates with specific chain lengths. Pioneering studies demonstrated that the product specificities of type I prenyltransferases were mainly determined by the amino acid residues at the 4th and 5th positions before the first aspartate-rich motif (FARM) of the prenyltransferases. We previously cloned a type I geranylgeranyl diphosphate synthase (GGDPSase) gene from Streptomyces griseolosporeus MF730-N6 [Hamano, Y., Dairi, T., Yamamoto, M., Kawasaki, T., Kaneda, K., Kuzuyama, T., Itoh, N., and Seto, H. (2001) BIOSCI: Biotechnol. Biochem. 65, 1627-1635]. In this study, a prenyltransferase gene was cloned from Streptomyces argenteolus A-2 and was confirmed to encode a type I farnesyl diphosphate synthase (FDPSase). Interestingly, the amino acid residues at the 4th and 5th positions before the FARM were the same in these two enzymes. To identify the amino acid that determines the product chain length, mutated enzymes, GGDPSase (L-50S), FDPSase (S-50L), GGDPSase (V-8A), FDPSase (A-8V), GGDPSase (A+57L), and FDPSase (L+58A), in which the amino acid residue at the -50th, -8th, and +57th (58th) position before or after the FARM was substi...Continue Reading

Citations

Aug 6, 2013·Plant Physiology and Biochemistry : PPB·Ram KulkarniVidya Gupta
May 20, 2003·European Journal of Biochemistry·Hisashi HemmiTokuzo Nishino
Aug 15, 2001·Antimicrobial Agents and Chemotherapy·I C MouraE A Kimura
Mar 10, 2005·Microbiology and Molecular Biology Reviews : MMBR·Daisuke UmenoFrances H Arnold
Dec 12, 2007·Bioscience, Biotechnology, and Biochemistry·Yutaka HayashiTohru Dairi
Sep 2, 2009·Insect Biochemistry and Molecular Biology·Sophie VandermotenBenoit Charloteaux
Aug 13, 2013·Journal of Hepatology·Philippe MetzRalf Bartenschlager
Dec 21, 2011·Microbes and Infection·Sandy Mattijssen, Ger J M Pruijn
Nov 19, 2004·Protein Engineering, Design & Selection : PEDS·Pyung Cheon LeeClaudia Schmidt-Dannert
Nov 18, 2005·Progress in Lipid Research·Florence BouvierBilal Camara

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