Interdigitation does not affect translational diffusion of lipids in liquid crystalline bilayers

Biophysical Journal
V Schram, T E Thompson

Abstract

Asymmetric phosphatidylcholine molecules with one acyl chain twice as long as the other, below their phase transition temperature, from a mixed interdigitated phase in which the longer acyl chain spans the entire bilayer. Experimental evidence in the literature suggests that, above their phase transition temperature, these molecules may still exhibit partial interdigitation, with the longer acyl chain extending partially into the opposite leaflet, and are packed more tightly than equivalent symmetric phosphatidylcholines. Using the fluorescence recovery after photobleaching technique, we have investigated the translational diffusion in multilayers of a liquid crystalline phase, asymmetric phosphatidylcholine, 1-stearoyl-2-capryl-phosphatidylcholine (C18C10PC). We used as a fluorescent probe either a phospholipid analog of the same acyl chain composition, NBD-C18C10PE, or the symmetric equivalent of the same molecular weight, N-(7-nitrobenzoxa-2,3-diazol-4-yl)-dimyristoyl-phosphatidyle thanolamine (NBD-DMPE). Translational diffusion coefficients were also determined by using both probes in multilayers of dimyristoyl-phosphatidylcholine (DMPC) and in the eutectic mixture DMPC/C18C10PC (40/60 mol). We found that in a given host li...Continue Reading

Citations

Feb 11, 2000·Chemistry and Physics of Lipids·Y H HaoJ W Chen
Apr 1, 2011·ACS Applied Materials & Interfaces·Peichi C HuNoah Malmstadt
Apr 6, 2011·The Journal of Physical Chemistry. B·Diego A PantanoPreston B Moore
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Jun 11, 2004·Biophysical Journal·Vincent Schram, Stephen B Hall
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Aug 1, 2007·Journal of the American Chemical Society·Giulio CaraccioloHeinz Amenitsch

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