Interdisciplinary study for the evaluation of biochemical alterations on mussel Mytilus galloprovincialis exposed to a tributyltin-polluted area

Analytical and Bioanalytical Chemistry
Emanuele MagiM Elisabetta Cosulich

Abstract

An interdisciplinary approach was employed to monitor the concentration and the effects of butyltin compounds in mussels (Mytilus galloprovincialis). Tissues from animals exposed to a marine area (Vado Ligure harbour) with a high concentration of tributyltin (TBT) were analysed and compared with control samples. TBT concentrations were measured by gas chromatography-mass spectrometry and the protein pattern in gill tissues was studied by proteomic analysis. Several proteomic signatures associated with contaminant exposure were observed; spots that were significantly increased in all contaminated samples were identified by mass spectrometry as fragments of β-tubulin. The degradation of β-tubulin was then confirmed by western blot analysis with specific anti-β-tubulin antibody. The effects observed on mussel gills after exposure in the TBT-polluted area are discussed.

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Citations

Sep 10, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Vittoria VentrellaAlessandra Pagliarani
Dec 3, 2009·Proteomics·Marco F L LemosAna C Esteves
Jan 26, 2018·Journal of Food Protection·Letizia AmbrosioLorella Severino

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