Interdomain hydrophobic interactions modulate the thermostability of microbial esterases from the hormone-sensitive lipase family.

The Journal of Biological Chemistry
Ping-Yi LiXi-Ying Zhang

Abstract

Microbial hormone-sensitive lipases (HSLs) contain a CAP domain and a catalytic domain. However, it remains unclear how the CAP domain interacts with the catalytic domain to maintain the stability of microbial HSLs. Here, we isolated an HSL esterase, E40, from a marine sedimental metagenomic library. E40 exhibited the maximal activity at 45 °C and was quite thermolabile, with a half-life of only 2 min at 40 °C, which may be an adaptation of E40 to the permanently cold sediment environment. The structure of E40 was solved to study its thermolability. Structural analysis showed that E40 lacks the interdomain hydrophobic interactions between loop 1 of the CAP domain and α7 of the catalytic domain compared with its thermostable homologs. Mutational analysis showed that the introduction of hydrophobic residues Trp(202) and Phe(203) in α7 significantly improved E40 stability and that a further introduction of hydrophobic residues in loop 1 made E40 more thermostable because of the formation of interdomain hydrophobic interactions. Altogether, the results indicate that the absence of interdomain hydrophobic interactions between loop 1 and α7 leads to the thermolability of E40. In addition, a comparative analysis of the structures of E...Continue Reading

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Citations

Mar 2, 2016·FEMS Microbiology Ecology·Lada E PetrovskayaElizaveta M Rivkina
Oct 19, 2016·Bioengineered·Lada E PetrovskayaElizaveta M Rivkina
Nov 22, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jie YangXiu-Lan Chen
Feb 27, 2017·Applied and Environmental Microbiology·Ping-Yi LiXi-Ying Zhang
Oct 12, 2018·Drug Development Research·Erik M Larsen, R Jeremy Johnson
Mar 24, 2017·Applied Microbiology and Biotechnology·Mariana Rangel PereiraAndrea Balan
Apr 4, 2020·Angewandte Chemie·Henrik MüllerUwe T Bornscheuer
Jan 8, 2021·Applied Biochemistry and Biotechnology·Noora BarzkarMahmoud Hafezieh
Dec 21, 2020·The FEBS Journal·Astrid HöppnerSander H J Smits
Jan 3, 2021·Progress in Lipid Research·Emeline RecazensDominique Langin
Mar 9, 2021·Computational and Structural Biotechnology Journal·Vega Miguel-RuanoJuan A Hermoso
Jun 1, 2021·The Journal of Biological Chemistry·Yi ZhangPing-Yi Li

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