Interdomain interface-mediated target recognition by the Scribble PDZ34 supramodule

The Biochemical Journal
Jinqi RenWei Feng

Abstract

Tandem-arranged PDZ [PSD-95 (postsynaptic density-95), Dlg (discs large homologue) and ZO-1 (zonula occludens-1)] domains often form structural and functional supramodules with distinct target-binding properties. In the present study, we found that the two PDZ domains within the PDZ34 tandem of Scribble, a cell polarity regulator, tightly pack in a 'front-to-back' mode to form a compact supramodule. Although PDZ4 contains a distorted αB/βB pocket, the attachment of PDZ4 to PDZ3 generates an unexpected interdomain pocket that is adjacent to and integrates with the canonical αB/βB pocket of PDZ3 to form an expanded target-binding groove. The structure of the PDZ34-target peptide complex further demonstrated that the peptide binds to this expanded target-binding groove with its upstream residues anchoring into the interdomain pocket directly. Mutations of the interdomain pocket and disruptions of the PDZ34 supramodule both interfere with its target-binding capacity. Therefore, the interdomain interface between the PDZ34 supramodule is intrinsically required for its target recognition and determines its target-binding specificity. This interdomain interface-mediated specific recognition may represent a novel mode of target recognit...Continue Reading

References

Apr 3, 2001·Annual Review of Neuroscience·M Sheng, C Sala
Dec 25, 2002·Human Molecular Genetics·Jennifer N MurdochAndrew J Copp
Mar 5, 2003·Journal of Molecular Biology·Jia-Fu LongMingjie Zhang
Apr 8, 2003·Structure·Beom Sik KangZygmunt S Derewenda
May 2, 2003·Nature·Mireille MontcouquiolMatthew W Kelley
Jul 16, 2003·Accounts of Chemical Research·Mingjie Zhang, Wenning Wang
Jun 9, 2004·Current Biology : CB·Stéphane AudebertJean-Paul Borg
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Dec 22, 2004·The Journal of Cell Biology·Jennifer ZeitlerDavid Bilder
Mar 19, 2005·The EMBO Journal·Olivier LahunaMicheline Misrahi
May 12, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mireille MontcouquiolMatthew W Kelley
Jun 2, 2006·The Journal of Biological Chemistry·Yingnan ZhangSachdev S Sidhu
Jun 23, 2006·Journal of Cellular Biochemistry·Laura M KallayLelita T Braiterman
Jul 29, 2006·Annual Review of Genetics·Iswar K Hariharan, David Bilder
Oct 28, 2006·Trends in Cell Biology·Patrick O HumbertSarah M Russell
Nov 4, 2006·Current Biology : CB·Naël OsmaniSandrine Etienne-Manneville
Dec 14, 2006·Acta Crystallographica. Section D, Biological Crystallography·Airlie J McCoy
Jul 17, 2007·International Review of Cytology·Lukas E Dow, Patrick O Humbert
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Tomoyuki Yamanaka, Shigeo Ohno
Aug 22, 2008·Human Molecular Genetics·Sébastien NolaMarie-Josée Santoni
Oct 3, 2008·PLoS Biology·Raffi TonikianSachdev S Sidhu
Nov 26, 2008·Oncogene·P O HumbertH E Richardson
Jan 21, 2009·Nature Reviews. Neuroscience·Wei Feng, Mingjie Zhang
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 2, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Lindsay RichierJames P Fawcett
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·T Geoff G BattyeAndrew G W Leslie
Aug 30, 2012·Essays in Biochemistry·Imogen ElsumHelena E Richardson
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ylva IvarssonPhilip M Kim

❮ Previous
Next ❯

Citations

Jul 11, 2016·Biomolecular NMR Assignments·Florent DelhommelFlorence Cordier
Apr 22, 2020·Advanced Therapeutics·Nikolaj R ChristensenKristian Strømgaard
Jun 30, 2019·The Journal of Cell Biology·Sahezeel AwadiaRafael Garcia-Mata
Oct 3, 2019·Scientific Reports·Eric M JanezicChris Hague

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.