PMID: 9433456Jan 20, 1998Paper

Interest of image processing in cell biology and immunology

Journal of Immunological Methods
S SabriPierre Bongrand

Abstract

Microscopy is a basic tool for cell biologists. Recent progress of electronics and computer science made powerful methodologies for digital processing of microscopic images easily available. These methods allowed impressive increase of the power of conventional microscopy. Dramatic image enhancement may be achieved by combination of filtering techniques, computer-based deblurring and contrast enhancement. Quantitative treatment of digitized images allows absolute determination of the density of different components of the observed sample, including antigens, intracellular calcium and pH. Morphometric studies are also greatly facilitated by image processing techniques. The capture of fast phenomena may be performed by transfer of small portion of microscopic images into computer memory as well as particular use of confocal microscopy. Finally, improved display of experimental data through coded colors or other procedures may enhance the amount of information that can be conveyed by visual examination of microscopical images. The purpose of the present review is to describe the basic principles of image processing and exemplify the power of this approach with a variety of illustrated applications to conventional, fluorescence or ...Continue Reading

References

Jan 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A W SmeuldersC J Cornelisse
Jan 1, 1979·Annual Review of Biophysics and Bioengineering·A S Waggoner
Feb 1, 1992·The Journal of Cell Biology·J R MonckJ M Fernandez
Feb 21, 1991·Nature·S R AdamsR Y Tsien
Nov 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L BenkoëlA Chamlian
Jan 1, 1989·Annual Review of Biophysics and Biophysical Chemistry·T M Jovin, D J Arndt-Jovin
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J A Connor
Apr 1, 1987·The Journal of Cell Biology·G R BrightD L Taylor
Sep 1, 1986·Cytometry·I T YoungB H Mayall
Jan 1, 1969·International Review of Cytology·E R Weibel
Aug 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·L Stryer, R P Haugland
Feb 1, 1984·The Journal of Cell Biology·L TanasugarnD L Taylor
Dec 1, 1984·The Journal of Cell Biology·M A Horwitz, F R Maxfield
Jan 1, 1984·Annual Review of Biophysics and Bioengineering·D A Agard
Feb 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L S BarakW W Webb
Nov 3, 1995·The Journal of Biological Chemistry·A PierresP Bongrand
Feb 1, 1993·Biophysical Journal·S KasasM R Celio

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Citations

Feb 5, 2000·Cell Motility and the Cytoskeleton·F RichelmeP Bongrand
Mar 30, 2000·International Journal of Cancer. Journal International Du Cancer·B AbdulkarimJ Bourhis
Oct 6, 1998·Journal of Immunological Methods·S J Merrill
Dec 18, 2001·Biosensors & Bioelectronics·Víctor H Pérez-LunaGabriel P López
Dec 25, 2007·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Karen Augustine-Rauch
Jan 13, 2006·Journal of Biotechnology·M C García-Malea LópezE Molina Grima
Oct 4, 2016·Injury·Esperanza Macarena López-PliegoJaime Domínguez
Jun 24, 2004·Toxicologic Pathology·Eugenia Floyd, Teresa M McShane
Jul 29, 2005·The International Journal of Artificial Organs·M MachinP Canu

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