PMID: 9630702Jun 19, 1998Paper

Interfacial activation, lysophospholipase and transacylase activity of group VI Ca2+-independent phospholipase A2

Biochimica Et Biophysica Acta
Y C Lio, E A Dennis

Abstract

The Group VI 80-kDa Ca2+-independent phospholipase A2 (iPLA2) has been purified from murine P388D1 macrophages and Chinese hamster ovary (CHO) cells. The amino acid sequence of the iPLA2 has been determined and shown to contain a lipase consensus sequence and eight ankyrin repeats, which makes it distinct from Group I-V PLA2s. This enzyme appears to play a key role in mediating basal phospholipid remodeling. We now report that the Group VI iPLA2 displays interfacial activation toward short chain phospholipids, 1-octanoyl-2-heptanoyl-sn-glycero-3-phosphocholine, 1,2-diheptanoyl-sn-glycero-3-phosphocholine, and 1,2-dihexanoyl-sn-glycero-3-phosphocholine micelles. ATP protects the iPLA2 from a loss in activity as a result of prolonged incubation during the assay. Hence higher enzyme activity is observed in the presence than in the absence of ATP. Similar protection was obtained with glycerol. In addition, the iPLA2 exhibits multiple activities which are strongly dependent on substrate presentation. The lysophospholipase activity of this enzyme was diminished by Triton X-100 and stimulated by glycerol. With the combination of 50 microM Triton X-100 and 50% glycerol, the enzyme's lysophospholipase activity achieved equivalent activi...Continue Reading

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Citations

Oct 22, 2008·Cardiovascular Drugs and Therapy·John E Burke, Edward A Dennis
May 29, 2004·Biochimica Et Biophysica Acta·Karin Killermann Lucas, Edward A Dennis
Mar 17, 2004·Biochemical and Biophysical Research Communications·Miki TamuraTeiji Sawa
Oct 12, 2000·International Journal for Parasitology·S CassaingH Chap
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Ichiro Kudo, Makoto Murakami
Nov 18, 2000·Biochimica Et Biophysica Acta·M V WinsteadE A Dennis
Jun 24, 2011·Chemical Reviews·Jonathan Z Long, Benjamin F Cravatt
Mar 2, 2010·Médecine sciences : M/S·Sylvain Baulande, Clotilde Langlois
May 20, 1999·Annual Review of Pharmacology and Toxicology·J BalsindeE A Dennis
Jan 25, 2002·Annual Review of Pharmacology and Toxicology·Camilla I Svensson, Tony L Yaksh
Mar 28, 2013·PloS One·Yuan-Hao HsuEdward A Dennis
Sep 21, 2001·Biochemical and Biophysical Research Communications·C H Chiu, S Jackowski
Jan 28, 2015·Proceedings of the National Academy of Sciences of the United States of America·Varnavas D MouchlisEdward A Dennis
Jan 18, 2016·Advances in Biological Regulation·Varnavas D Mouchlis, Edward A Dennis
Mar 9, 2011·Progress in Lipid Research·Martin HermanssonPentti Somerharju
Apr 14, 2016·Chemico-biological Interactions·Daniel K Nomura, John E Casida
Jan 9, 2013·Expert Opinion on Therapeutic Patents·Victoria Magrioti, George Kokotos
Apr 19, 2015·Advances in Colloid and Interface Science·Srinivasan Damodaran
Sep 6, 2000·Prostaglandins, Leukotrienes, and Essential Fatty Acids·C N Bennett, D F Horrobin
Apr 22, 2015·PloS One·Randi M SommerfeltBerit Johansen
Apr 20, 2000·The Journal of Investigative Dermatology·E MauryH Chap
May 30, 2015·Journal of Lipid Research·Sasanka RamanadhamXiaoyong Lei
Apr 3, 2002·The Journal of Biological Chemistry·Marianne van TienhovenPaul Glynn
Aug 25, 2016·The Journal of Biological Chemistry·Edward A Dennis
Feb 26, 2015·The Journal of Biological Chemistry·Krishna Chaithanya BatchuPentti Somerharju
Nov 18, 2000·Biochimica Et Biophysica Acta·D A Six, E A Dennis
Jul 9, 1999·Biochimica Et Biophysica Acta·A Wang, E A Dennis
May 14, 2003·Cellular Signalling·Sajal Chakraborti
Sep 3, 2005·Biochimica Et Biophysica Acta·Paul Glynn
Sep 16, 2006·Biochimica Et Biophysica Acta·Ralph H Schaloske, Edward A Dennis

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