Interfacial complexes between a protein and lipophilic ions at an oil-water interface

Analytical Chemistry
Rune Andersen HartvigHenrik Jensen

Abstract

The interaction between an intact protein and two lipophilic ions at an oil-water interface has been investigated using cyclic voltammetry, impedance based techniques and a newly developed method in which the biphasic oil-water system is analyzed by biphasic electrospray ionization mass spectrometry (BESI-MS), using a dual-channel electrospray emitter. It is found that the protein forms interfacial complexes with the lipophilic ions and that it specifically requires the presence of the oil-water interface to be formed under the experimental conditions. Furthermore, impedance based techniques and BESI-MS with a common ion to polarize the interface indicated that the Galvani potential difference across the oil-water interface significantly influences the interfacial complexation degree. The ability to investigate protein-ligand complexes formed at polarized liquid-liquid interfaces is thus a new analytical method for assessing potential dependent interfacial complexation using a structure elucidating detection principle.

Citations

Mar 19, 2013·Analytica Chimica Acta·Grégoire Herzog, Valerio Beni
Oct 4, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Manuel A MéndezHubert H Girault
Jan 28, 2016·The Journal of Physical Chemistry. B·Jana HladílkováMikael Lund
Mar 8, 2016·The Journal of Physical Chemistry. B·Mahreen AroojRicardo L Mancera
Jul 26, 2012·Chemistry, an Asian Journal·Eva Alvarez de EulateDamien W M Arrigan

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