Interfacial dilational properties of tea polyphenols and milk proteins with gut epithelia and the role of mucus in nutrient adsorption

Food & Function
Anilda GuriMilena Corredig

Abstract

By interacting with nutrients, the mucus layer covering the intestinal epithelium may mediate absorption. This study aimed to determine possible interactions between epigallocatechin-3-gallate (EGCG), skim milk proteins or their complexes with human intestinal mucin films. The films were extracted from postconfluent monolayers of HT29-MTX, a human intestinal cell line, and a model system was created using drop shape tensiometry. The EGCG uptake tested in vitro on postconfluent Caco-2 cells or co-cultures of Caco-2/HT29-MTX (mucus producing) showed recovery of bioavailable EGCG only for Caco-2 cell monolayers, suggesting an effect of mucus on absorption. Interfacial dilational rheology was employed to characterize the properties of the interface mixed with mucus dispersion. Adsorption of polyphenols greatly enhanced the viscoelastic modulus of the mucus film, showing the presence of interactions between the nutrient molecules and mucus films. On the other hand, in situ digestion of milk proteins using trypsin showed higher surface activities as a result of protein unfolding and competitive adsorption of the hydrolyzed products. There was an increase of viscoelastic modulus over the drop ageing time for the mixed interfaces, indi...Continue Reading

References

Feb 1, 1982·Journal of Oral Pathology·L A TabakS A Ellison
Dec 5, 2000·Journal of Dairy Science·J M GirardetJ L Gaillard
Feb 21, 2002·Journal of Agricultural and Food Chemistry·Mariken J T J ArtsAalt Bast
Jul 5, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Anders Bennick
Apr 19, 2003·Journal of Colloid and Interface Science·Anneke H MartinTon van Vliet
May 11, 2004·Biomacromolecules·Elisabeth JöbstlMike P Williamson
Nov 16, 2004·International Journal of Pharmaceutics·Li ZhangZhong Zuo
Aug 1, 2006·Phytochemistry·Dale G NagleYu-Dong Zhou
Oct 2, 2008·Journal of Agricultural and Food Chemistry·V Aguié-BéghinR Douillard
Jan 27, 2009·Advanced Drug Delivery Reviews·Samuel K LaiJustin Hanes
Mar 1, 2012·Journal of Agricultural and Food Chemistry·Eleanor M D'AgostinoGleb E Yakubov
Sep 15, 2012·Trends in Biotechnology·Aurélie GuerraMonique Alric
Apr 16, 2013·Colloids and Surfaces. B, Biointerfaces·Damiano RossettiLibero Liggieri
May 21, 2013·Colloids and Surfaces. B, Biointerfaces·Damiano RossettiLibero Liggieri
Sep 5, 2013·Journal of Photochemistry and Photobiology. B, Biology·Philippe BourassaHeidar-Ali Tajmir-Riahi
Jan 15, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Marie BoeghHanne M Nielsen
Aug 1, 2014·Journal of Agricultural and Food Chemistry·Pauline LestringantMilena Corredig
Aug 1, 2011·Food Chemistry·C D KanakisHeidar-Ali Tajmir-Riahi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.