Interfacial properties of amphipathic beta strand consensus peptides of apolipoprotein B at oil/water interfaces.

Journal of Lipid Research
Libo Wang, Donald M Small

Abstract

The region between residues 968 and 1882 of apolipoprotein B (apoB-21 to apoB-41) is rich in amphipathic beta strands (AbetaSs) and promotes the assembly of primordial triacylglyceride (TAG)-rich lipoproteins. To understand the importance of AbetaS in recruiting TAG, the interfacial properties of two AbetaS consensus peptides, P12 and P27, were studied at dodecane/water (DD/W) and triolein/water (TO/W) interfaces. P12 (acetyl-LSLSLNADLRLK-amide) and P27 (acetyl-LSLSLNADLRLKNGNLSLSLNADLRLK-amide), when added into the aqueous phase surrounding a suspended oil drop (dodecane or triolein), decreased the interfacial tension (gamma) in a concentration-dependent manner. At the DD/W interface, 1 x 10(-5) M P12 decreased gamma to approximately 20 mN/m and 6.6 x 10(-6) M P27 decreased gamma to approximately 13 mN/m. At the TO/W interface, 1.5 x 10(-5) M P12 decreased gamma to approximately 14 mN/m and 9.0 x 10(-6) M P27 decreased gamma to approximately 12 mN/m. The surface area of both peptides was between 11.2 and 15.1 angstroms2 per residue, consistent with beta sheets lying flat on DD/W and TO/W interfaces. P12 and P27 are almost purely elastic on DD/W, TO/W, and air/water interfaces. When P12 and P27 were compressed beyond the equili...Continue Reading

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H HerscovitzD M Small
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·D M EngelmanA Goldman
Jan 1, 1986·Annual Review of Biophysics and Biophysical Chemistry·D Atkinson, D M Small
Jan 31, 1995·Proceedings of the National Academy of Sciences of the United States of America·H HerscovitzD M Small
Apr 5, 2000·The Journal of Biological Chemistry·W L Burch, H Herscovitz
Jul 14, 2000·The Journal of Biological Chemistry·J ReadC C Shoulders
Aug 9, 2002·Journal of the American Chemical Society·Hanna RapaportDavid A Tirrell
Mar 19, 2003·Current Opinion in Lipidology·Alberto ZambonJohn D Brunzell
Aug 28, 2003·The Journal of Biological Chemistry·Gregory S ShelnessRichard B Weinberg

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Citations

May 14, 2005·Current Opinion in Lipidology·Olga Gursky
May 14, 2005·Current Opinion in Lipidology·Gregory S Shelness, Aubrey S Ledford
Apr 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Libo WangDonald M Small
Mar 21, 2007·Biophysical Journal·Zhenghui Gordon JiangC James McKnight
Sep 16, 2008·Biochimica Et Biophysica Acta·Shin-ya MoritaTetsurou Handa
Nov 7, 2016·Biochimica Et Biophysica Acta. Biomembranes·Scott M GordonAlan T Remaley
Dec 7, 2006·Journal of Lipid Research·Marcel M W SmolenaarsDick J Van der Horst
Jan 13, 2006·The Journal of Biological Chemistry·Aubrey S LedfordGregory S Shelness
May 11, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Iván López-MonteroFrancisco Monroy
Jan 17, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Matthew A MitscheDonald M Small
May 4, 2021·The Journal of Physical Chemistry. B·Mona MirheydariEdgar E Kooijman
Dec 15, 2010·The Journal of Physical Chemistry. B·Vladimir Vaiser, Hanna Rapaport

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