Interfacial recognition of human prostate-specific antigen by immobilized monoclonal antibody: effects of solution conditions and surface chemistry.

Journal of the Royal Society, Interface
XiuBo ZhaoJian R Lu

Abstract

The specific recognition between monoclonal antibody (anti-human prostate-specific antigen, anti-hPSA) and its antigen (human prostate-specific antigen, hPSA) has promising applications in prostate cancer diagnostics and other biosensor applications. However, because of steric constraints associated with interfacial packing and molecular orientations, the binding efficiency is often very low. In this study, spectroscopic ellipsometry and neutron reflection have been used to investigate how solution pH, salt concentration and surface chemistry affect antibody adsorption and subsequent antigen binding. The adsorbed amount of antibody was found to vary with pH and the maximum adsorption occurred between pH 5 and 6, close to the isoelectric point of the antibody. By contrast, the highest antigen binding efficiency occurred close to the neutral pH. Increasing the ionic strength reduced antibody adsorbed amount at the silica-water interface but had little effect on antigen binding. Further studies of antibody adsorption on hydrophobic C8 (octyltrimethoxysilane) surface and chemical attachment of antibody on (3-mercaptopropyl)trimethoxysilane/4-maleimidobutyric acid N-hydroxysuccinimide ester-modified surface have also been undertaken...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E W SilvertonD R Davies
Jan 1, 1991·Journal of Biomaterials Science. Polymer Edition·D R LuK Park
Jan 1, 1997·Biosensors & Bioelectronics·L C Shriver-LakeF S Ligler
Dec 16, 1998·Journal of Colloid and Interface Science·T J SuJ Penfold
Oct 22, 2003·Biosensors & Bioelectronics·Lorena TedeschiAndrea Mencaglia
Apr 6, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Jian R LuJeff Penfold
Jun 9, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Sang Wook OhEui Yul Choi
Jul 9, 2005·Biosensors & Bioelectronics·Peng WuDavid W Grainger
Jan 9, 2007·Colloids and Surfaces. B, Biointerfaces·Jongwon ParkKazuhiko Ishihara
Dec 18, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Luke A ConnalGreg G Qiao
Mar 5, 2008·European Biophysics Journal : EBJ·Anton P Le BrunJeremy H Lakey
Aug 7, 2009·Journal of the Royal Society, Interface·XiuBo ZhaoJian R Lu
Nov 26, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Fang PanJohn R P Webster
Apr 24, 2010·Analytical Chemistry·Rachel D LoweNicolas H Voelcker
Apr 27, 2010·Analytical Chemistry·Takahisa IbiiTakeshi Imamura
May 27, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Xiubo ZhaoJikui Luo

❮ Previous
Next ❯

Citations

Mar 16, 2013·Marine Drugs·María J Hortigüela, J Gerard Wall
Apr 8, 2015·Journal of Pharmaceutical Sciences·Tatiana PerevozchikovaChristopher J Roberts
Aug 12, 2014·Bioconjugate Chemistry·Eduardo ZappIolanda Cruz Vieira
Dec 23, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Khai T LeeSimon R Corrie
Jan 23, 2013·The Analyst·Anke K TrillingHan Zuilhof
Mar 28, 2020·Pharmaceutics·Xinyue Huang, Helen E Townley
May 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peter HollowellJian R Lu
Apr 16, 2017·Journal of Chromatography. a·Alice R MazzerDaniel G Bracewell
Dec 8, 2017·ACS Applied Materials & Interfaces·Fang PanJian Ren Lu
Dec 1, 2018·ACS Applied Materials & Interfaces·Zongyi LiJian Ren Lu
Mar 8, 2019·Bioconjugate Chemistry·Guadalupe RuizJeremy D Driskell
Sep 25, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Fang PanXiubo Zhao

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.