Interfacial rheology of blood proteins adsorbed to the aqueous-buffer/air interface

Biomaterials
Florly S AriolaErwin A Vogler

Abstract

Concentration-dependent, interfacial-shear rheology and interfacial tension of albumin, IgG, fibrinogen, and IgM adsorbed to the aqueous-buffer/air surface is interpreted in terms of a single viscoelastic layer for albumin but multi-layers for the larger proteins. Two-dimensional (2D) storage and loss moduli G(') and G(''), respectively, rise and fall as a function of bulk-solution concentration, signaling formation of a network of interacting protein molecules at the surface with viscoelastic properties. Over the same concentration range, interfacial spreading pressure Pi(LV) identical with gamma(lv)(o)-gamma(lv) rises to a sustained maximum Pi(LV)(max). Mixing as little as 25 w/v% albumin into IgG at fixed total protein concentration substantially reduces peak G('), strongly suggesting that albumin acts as rheological modifier by intercalating with adsorbed IgG molecules. By contrast to purified-protein solutions, serially diluted human blood serum shows no resolvable concentration-dependent G(')and G('').

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Citations

Sep 13, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Suvajyoti GuhaMichael R Zachariah
Sep 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Zhengzheng LiaoIvan J Dmochowski
Apr 23, 2010·Biointerphases·Cyrille VézyAndreas R Bausch
Jul 27, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Konstantin GolemanovSimeon D Stoyanov
May 1, 2013·Journal of Colloid and Interface Science·Suvajyoti GuhaMichael R Zachariah
Jan 27, 2009·Biomaterials·Erwin A Vogler, Christopher A Siedlecki
Jan 13, 2009·Acta Biomaterialia·Evan S LeibnerErwin A Vogler
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Nov 18, 2011·Biomaterials·Erwin A Vogler
Jul 24, 2007·Biomaterials·Rui ZhuoErwin A Vogler
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Jan 31, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Paul ChaErwin A Vogler
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