PMID: 16615670Apr 18, 2006Paper

Interference by venlafaxine ingestion in the detection of tramadol by liquid chromatography linked to tandem mass spectrometry for the screening of illicit drugs in human urine

Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists
K R Allen

Abstract

Confirmation of the presence of illicit drugs in human fluids by liquid chromatography linked to atmospheric pressure ionisation tandem mass spectrometry (LC/MS/MS) is becoming increasingly popular. A rapid LC/MS/MS method is described using reversed phase gradient elution chromatography on a 50 x 3 mm base deactivated C8 column with identification of drugs based on their Multiple Reaction monitoring (MRM) transitions, retention time, and co-elution of stable isotopic analogues where available. The method is used for drug confirmation following initial screening by immunoassay, and identification of drugs such as tramadol, for which no immunoassay is available. False positive results for tramadol were observed in cases where subjects were being treated with the commonly prescribed antidepressant drug venlafaxine. This was a result of the presence of the metabolite O-desmethylvenlafaxine, which has a similar transition to tramadol. This study highlights the need to consider drug metabolites, as well as parent drugs in interferences in LC/MS/MS methods.

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Citations

Oct 9, 2012·Clinical Biochemistry·Andrea Božović, Vathany Kulasingam
Aug 16, 2011·Forensic Science International : Synergy·Maria KingbäckFredrik C Kugelberg
Dec 15, 2010·Biomedical Chromatography : BMC·Lewis Couchman, Phillip E Morgan
May 11, 2012·Drug Testing and Analysis·Eva SaarOlaf H Drummer
May 11, 2016·Clinical Biochemistry·Maria Shipkova, Dobrin Svinarov
Jan 8, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·John M VeranthGarold S Yost
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Mar 23, 2007·Analytical and Bioanalytical Chemistry·Hans H Maurer
May 24, 2007·Analytical and Bioanalytical Chemistry·Franck Saint-MarcouxPierre Marquet
Aug 1, 2009·Bioanalysis·Gary M ReisfieldRoger L Bertholf
Feb 1, 2020·International Anesthesiology Clinics·A Zara HerskovitsLynne Uhl
Oct 5, 2017·Journal of Analytical Toxicology·Ana Celia Muñoz-MuñozLawrence Andrade
May 18, 2007·Analytical Chemistry·T A BrettellJ R Almirall
Jan 1, 2018·The Journal of Applied Laboratory Medicine·Darlington DansoPaul J Jannetto

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