Interference in immunoassays by human IgM with specificity for the carbohydrate moiety of animal proteins

Journal of Immunological Methods
Justus AdédoyinMarianne van Hage

Abstract

In allergen characterization common immunoassays such as ELISA and immunoblotting are often used to evaluate human IgE binding of sera from allergic individuals. In an attempt to find and characterize new cat allergens, heavily stained bands were found at 67 and 90 kDa on blotted cat dander extract. Further characterization confirmed these bands to be cat IgA and IgM heavy chains. Analyses in ELISA of purified cat immunoglobulins linked the human IgE binding to cat IgA and IgM and also revealed complete mutual cross-reactivity between the two isotypes. IgE reactivity was found to be directed to carbohydrates of the immunoglobulins and to IgM from 7 out of 9 animal species, but not to human immunoglobulins. Further investigations revealed that the reaction was not mediated by human IgE but by a factor in the serum cross-linking animal immunoglobulins and alkaline phosphatase. The factor was further studied and shown to be human IgM. This IgM fits into the heterophile antibody classification and is directed against carbohydrates on animal immunoglobulins and on calf intestine alkaline phosphatase.

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Citations

Jan 17, 2012·Immunology and Allergy Clinics of North America·Madhan MasilamaniWayne Shreffler
Apr 14, 2009·The Journal of Allergy and Clinical Immunology·Hans GrönlundMarianne van Hage
Mar 6, 2007·The Journal of Allergy and Clinical Immunology·Justus AdédoyinMarianne van Hage
Nov 15, 2014·Allergy·C LambertUNKNOWN AllergoBioNet sIgE accreditation interest group
Apr 23, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A Gonzalez-QuintelaA Linneberg
Oct 13, 2006·International Archives of Allergy and Immunology·Friedrich Altmann
Jun 8, 2013·Current Opinion in Allergy and Clinical Immunology·Scott P Commins, Thomas A E Platts-Mills
Dec 16, 2006·The Journal of Biological Chemistry·Guro GafvelinJuha Punnonen
May 7, 2020·The World Allergy Organization Journal·Luis CaraballoAlexander Karaulov
May 25, 2021·World Journal of Methodology·Florin-Dan PopescuMariana Vieru

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