Interference of antisperm antibodies with the induction of the acrosome reaction by zona pellucida (ZP) and its relationship with the inhibition of ZP binding

Fertility and Sterility
F FrancavillaG Ruvolo

Abstract

To determine whether antisperm antibodies can interfere with the induction of the acrosome reaction (AR) by the zona pellucida (ZP) and whether this interference also can occur in the absence of an inhibitory effect on ZP binding. Prospective in vitro study. A tertiary care center, the Andrologic Clinic, University of L'Aquila. Sera from 12 infertile patients with high titers of circulating antibodies directed against the sperm head were studied. None The effect of antisperm antibodies on ZP binding was evaluated by matching antibody-exposed and nonexposed donor sperm suspensions labeled with fluorescein or rhodamine, respectively, and incubated with the same salt-stored human ZPs. The effect of antibodies on ZP-induced AR was determined by challenging antibody-exposed and nonexposed donor sperm suspensions with human ZPs disaggregated with acidic NaH2PO4. Acrosomal status was evaluated using fluorescein-labeled Pisum sativum agglutinin and supravital stain Hoechst 33258. In some selected cases, the effect of antisperm antibodies on the acrosomal status of sperm bound to intact ZP also was evaluated using transmission electron microscopy. Five of 12 sera exhibited an inhibitory effect on ZP binding. An inhibition of AR inductio...Continue Reading

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Citations

Jun 14, 2000·Animal Reproduction Science·D D VarnerL Johnson
Dec 29, 2000·Andrologia·T ZeginiadouS Mantalenakis
Nov 26, 1999·Clinical Obstetrics and Gynecology·B J Van Voorhis, A E Sparks
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Jul 19, 2013·Human Fertility : Journal of the British Fertility Society·Mathew TomlinsonUNKNOWN British Fertility Society
Oct 24, 2009·Fertility and Sterility·Carolina VeauteMónica H Vazquez-Levin
Jun 14, 2005·American Journal of Reproductive Immunology : AJRI·Sanja VujisićBozica Sokolić
Nov 25, 2003·American Journal of Reproductive Immunology : AJRI·Clara I Marín-BriggilerJorge G Tezón
Sep 16, 2006·Immunological Reviews·Monika Fijak, Andreas Meinhardt
May 19, 2006·Reproductive Medicine and Biology·Masaharu KamadaTakahide Mori

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