PMID: 2502577Jul 6, 1989Paper

Interference of complement with the binding of carcinoembryonic antigen to solid-phase monoclonal antibodies

Journal of Immunological Methods
O P Börmer

Abstract

Six mouse monoclonal antibodies against carcinoembryonic antigen were evaluated for use in a solid-phase immunometric assay. Three IgG2 antibodies, when attached to polymer particles or microtiter wells, were found to be severely inhibited by fresh serum. The remaining three antibodies, which were of the IgG1 subclass, were inhibited only slightly or not at all when used in the same way. With the aid of labelled antibodies against C1q and C3, it was shown that antibody inhibition was accompanied by the binding of large amounts of these complement factors. Experiments involving heat inactivation or dilutions of serum, or the addition of EDTA, consistently revealed the same correlation between binding of complement factors and inhibition of CEA binding to antibody. It is suggested that the significant inhibition of the solid-phase IgG2 antibodies was caused by classical pathway activation of complement by the solid-phase antibody even in the absence of antigen. The slight inhibition of solid-phase IgG1 antibodies observed at high serum concentrations was probably due to complement binding by the alternative pathway. Preliminary evidence suggests that complement interference is not restricted to CEA assays, but is a potential prob...Continue Reading

References

Feb 28, 1978·Biochemical and Biophysical Research Communications·P J Fraker, J C Speck
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Citations

Aug 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J HellmanK Pettersson
Oct 19, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Kristina ScheeElisabeth Paus
Mar 9, 1990·Journal of Immunological Methods·O P Börmer, K Nustad
Dec 9, 2003·Journal of Immunological Methods·Elisabeth PausDavid J Warren
Apr 6, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Johan BjernerKjell Nustad
Sep 9, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Nils BolstadKjell Nustad
May 17, 2014·Bioanalysis·Boris GorovitsSheldon Leung
Apr 22, 2009·Journal of Immunological Methods·Tuomas NäreojaHarri Härmä
Jun 30, 2006·Clinical Biochemistry·Johanna YlikotilaKim Pettersson
Apr 12, 2005·Clinical Biochemistry·Johan BjernerKjell Nustad
Oct 18, 2006·Critical Reviews in Clinical Laboratory Sciences·Susann ErikssonKim Pettersson
Aug 6, 2005·Scandinavian Journal of Clinical and Laboratory Investigation·J Bjerner
Feb 2, 2013·Clinical Biochemistry·Heidi HyytiäKim Pettersson
Feb 1, 1993·Scandinavian Journal of Clinical and Laboratory Investigation·O P Børmer
Jan 1, 1991·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·W G Wood
Jan 1, 1991·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·O P BørmerK Nustad
Aug 1, 2006·Human Reproduction Update·Ulf-Håkan StenmanLeena Valmu
Jul 29, 1998·Annals of Clinical Biochemistry·C M Sturgeon, E J McAllister

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