Interference of hemoglobin Hope on beta-thalassemia diagnosis by the capillary electrophoresis Method

American Journal of Clinical Pathology
Sitthichai PanyasaiSakorn Pornprasert

Abstract

The β-chain hemoglobin (Hb) variants interfere with the diagnosis of β-thalassemia trait using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). We analyzed the effect of Hb Hope, a β-chain Hb variant frequently found in the Thai population, on β-thalassemia trait diagnosis. HPLC and CE were used to quantify the level of HbA(2) in 11 whole blood samples containing Hb Hope. The levels of Hb Hope detected by both methods were similar. An elevated HbA(2) level was found in all samples analyzed by the CE method, while 1 was increased when analyzed by HPLC, which was a compound heterozygous of Hb Hope and α-thalassemia-1 SEA-type deletion. Of 11 samples, 6 had mean corpuscular volumes within the reference range. All samples showed negative results for molecular analysis of β(0)-thalassemia codon 17, 41/42, and 71/72 mutations and β-thalassemia 3.5-kb deletion. Therefore, Hb Hope interfered with the diagnosis of β-thalassemia trait analyzed by CE but not by HPLC.

References

Jan 1, 1976·Hemoglobin·S PootrakulS Na-Nakorn
Oct 21, 2000·Bone Marrow Transplantation·V S TanphaichitrH Ideguchi
Jul 26, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Margaret Jenkins, Sujiva Ratnaike
Aug 25, 2004·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·S SirichotiyakulT Tongsong
Oct 14, 2004·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·Yohan RobinsonDieter Böning
Dec 11, 2008·European Journal of Haematology·Prathom PrathomtanapongT Sanguansermsri
Oct 30, 2009·American Journal of Clinical Pathology·Daniel David MaisDavid F Keren

Citations

Jul 23, 2015·Laboratory Medicine·Sutirtha ChakrabortyPrasad Krishnan
Feb 18, 2012·American Journal of Clinical Pathology·Monica V E GallivanChing-Nan Ou
Jul 14, 2016·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Sitthichai Panyasai, Sakorn Pornprasert
Nov 19, 2011·American Journal of Clinical Pathology·David F Keren, Lynn Rae Sample

Related Concepts

Hemoglobin Hope
High Pressure Liquid Chromatography Procedure
Abnormal Hemoglobins
Genetic Carriers
Thalassemia Minor
Electrophoresis, Capillary
Korean Race
Beta Thalassemia
High Pressure Liquid Chromatography Procedure
Gene Deletion

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.