Interfering Effects of In Vitro Fertilization and Vitrification on Expression of Gtl2 and Dlk1 in Mouse Blastocysts

International Journal of Fertility & Sterility
Elham MovahedMohammad Salehi

Abstract

Embryo vitrification is a key instrument in assisted reproductive technologies (ARTs). However, there is increasing concern that vitrification adversely affects embryo development. This study intends to assess the effect of vitrification on developmental competence, in addition to expressions of long non-coding RNA (lncRNA) gene trap locus 2 (Gtl2) and its reciprocal imprinted gene delta-like homolog 1 (Dlk1), in mouse blastocysts. In this experimental study, we have designed three experimental groups: control (fresh blastocysts collected from superovulated mice), in vitro fertilization (IVF; blastocysts derived from IVF) and vitrification (IVF derived blastocysts subjected to vitrification/warming at the 2-cell stage). Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to assess the expression levels of Gtl2 and Dlk1 in the blastocysts. The results showed that vitrification group had significantly lower blastocyst and hatching rates compared to the IVF group (P<0.037) and (P<0.041), respectively. Gtl2 was down-regulated and Dlk1 was up-regulated following the IVF and vitrification (P<0.05). These results suggested that IVF and vitrification disturbed genomic imprinting and lncRNA gene expressi...Continue Reading

Citations

Jun 4, 2021·Systems Biology in Reproductive Medicine·Rossella CannarellaAldo E Calogero

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Methods Mentioned

BETA
Gene
reverse-transcription PCR
PCR
reverse transcription PCR
gene trap

Software Mentioned

REST
Relative Expression Software Tool ( REST
Statistical Package for the Social Science software

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