Interfering histone deacetylase 4 inhibits the proliferation of vascular smooth muscle cells via regulating MEG3/miR-125a-5p/IRF1

Cell Adhesion & Migration
Xiangtao ZhengMengtao Zhou

Abstract

In this study, we investigated the role ofhistone deacetylase 4 (HDAC4) and MEG3/miR-125a-5p/interferonregulatoryfactor 1 (IRF1) on vascular smooth muscle cell (VSMCs)proliferation. Platelet derived growth factor (PDGF)-BB was used toinduce the proliferation and migration of VSMCs. The expressionsof MEG3, miR-125a-5p, HDAC4 and IRF1in VSMCs were detectedby qRT-PCR and western blot, respectively. ChIP assay was usedto determine the relationship between MEG3 and HDAC4. Doubleluciferase reporter assay was used to test the regulation betweenmiR-125-5p and IRF1. Results showed that PDGF-BB decreasedthe expression of MEG3 and IRF1, while increased the expressionof miR-125a-5p and HDAC4. In addition, HDAC4 knockdowninhibited the proliferation and migration of VSMCs via upregulatingMEG3 and downregulating miR-125a-5p. MiR-125a-5p inhibitorcould repress the proliferation and migration of VSMCs andalleviate intimal hyperplasia (IH) by directly upregulating IRF1expression. These results suggested that HDAC4 interferenceinhibited PDGF-BB-induced VSMCs proliferation via regulatingMEG3/miR-125a-5p/IRF1 axis, and then alleviated IH.

References

Dec 6, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Yiming LinYuqing E Chen
Mar 1, 2012·The Biochemical Journal·Roman GinnanHarold A Singer
Mar 7, 2012·Journal of Molecular Endocrinology·Yunli ZhouAnne Klibanski
Feb 5, 2016·Biochemical and Biophysical Research Communications·Gui-Zhen QiuBan Liu
Oct 25, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jian-Qin LiLi-Juan Cao
May 16, 2017·Journal of Molecular Biology·C GareriC Indolfi
Aug 30, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zengxian SunYun Liu
Feb 7, 2018·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Matthew C HymanDavid S Frankel
Feb 24, 2018·Scientific Reports·Nicoletta MarroncelliViviana Moresi

❮ Previous
Next ❯

Citations

Feb 6, 2020·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Xiaoying Jiang, Qilan Ning
May 8, 2020·International Journal of Molecular Sciences·Duk-Hwa KwonHyun Kook
Dec 8, 2020·Frontiers in Cell and Developmental Biology·Xiaona ChenHong Li
Aug 18, 2021·BMC Cardiovascular Disorders·Adeleh PoursalehMohammad Najafi
Jul 17, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Ekhtear HossainMadhu B Anand-Srivastava

❮ Previous
Next ❯

Methods Mentioned

BETA
histone acetylation
enzymatic dissociation
PCR
Protein Assay
immunoprecipitation
ChIP
transfection

Software Mentioned

Digimizer

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.