Interferon-γ suppresses intestinal epithelial aquaporin-1 expression via Janus kinase and STAT3 activation

PloS One
Michael S DicayWallace K MacNaughton

Abstract

Inflammatory bowel diseases are associated with dysregulated electrolyte and water transport and resultant diarrhea. Aquaporins are transmembrane proteins that function as water channels in intestinal epithelial cells. We investigated the effect of the inflammatory cytokine, interferon-γ, which is a major player in inflammatory bowel diseases, on aquaporin-1 expression in a mouse colonic epithelial cell line, CMT93. CMT93 monolayers were exposed to 10 ng/mL interferon-γ and aquaporin-1 mRNA and protein expressions were measured by real-time PCR and western blot, respectively. In other experiments, CMT93 cells were pretreated with inhibitors or were transfected with siRNA to block the effects of Janus kinases, STATs 1 and 3, or interferon regulatory factor 2, prior to treatment with interferon-γ. Interferon-γ decreased aquaporin-1 expression in mouse intestinal epithelial cells in a manner that did not depend on the classical STAT1/JAK2/IRF-1 pathway, but rather, on an alternate Janus kinase (likely JAK1) as well as on STAT3. The pro-inflammatory cytokine, interferon-γ may contribute to diarrhea associated with intestinal inflammation in part through regulation of the epithelial aquaporin-1 water channel via a non-classical JAK/...Continue Reading

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Citations

Sep 3, 2016·International Journal of Molecular Sciences·Cui ZhuZongyong Jiang
May 22, 2020·Inflammation·Lezy Flora Mariajoseph-AntonyChidambaram Prahalathan
Mar 6, 2018·Frontiers in Physiology·Rosaria MeliAlessandra Pelagalli
Aug 10, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Michael A PeplowskiWallace K MacNaughton
Apr 16, 2021·Investigative Ophthalmology & Visual Science·Dominique GeoffrionMona Harissi-Dagher
Jul 3, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Liang WangDapeng Chen

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Methods Mentioned

BETA
PCR
Protein Assay
Transfection

Software Mentioned

GraphPad Prism
Quantity One
ImageJ

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