PMID: 15223777Jun 30, 2004Paper

Interferon alpha 2b decreases hepatic micrometastasis in a murine model of ocular melanoma by activation of intrinsic hepatic natural killer cells

Investigative Ophthalmology & Visual Science
Hua YangHans E Grossniklaus

Abstract

To investigate in a murine model the mechanism by which micrometastatic melanoma, which spreads from the eye to the liver, is controlled by interferon (IFN)-alpha 2b. Major histocompatibility (MHC) class I antigen (H-2, all haplotypes) expression in three murine melanoma cell lines (Queens, B16LS9, B16F10) was determined by flow cytometric immunophenotyping. The cell lines were heterotopically inoculated into the posterior compartments (PCs) of C57Bl/6 mice, and the mice were given intraperitoneal (IP) injections of IFN-alpha 2b or PBS for 1 or 4 days before enucleation at 7 days after inoculation. Groups of mice were made NK deficient or depleted with subcutaneous (s.c.) injection of anti-asialo GM1. The mice were killed at 28 days or 56 days (survival experiment) after inoculation, and the number of hepatic micrometastases was histologically determined. NK cells were isolated from the spleen and liver at necropsy, and propidium iodide labeled target-specific cytolysis was determined by flow cytometry. The micrometastases were evaluated for apoptosis and proliferation with TUNEL and MIB1 immunostaining, respectively, and TUNEL-to-MIB1 ratios were determined. Hepatic NK cells were immunostained with CD49b. MHC class I antigen w...Continue Reading

Citations

Mar 14, 2008·Melanoma Research·Hua YangHans E Grossniklaus
Jun 27, 2007·Investigative Ophthalmology & Visual Science·Robert FolbergAndrew J Maniotis
Sep 12, 2009·Ophthalmology·Anne Marie LaneEvangelos S Gragoudas
Oct 25, 2016·Human Pathology·Hans E GrossniklausSarah E Coupland
Jul 11, 2006·Medical Hypotheses·William Harless, Yang Qiu
Mar 4, 2010·International Journal of Cancer. Journal International Du Cancer·Matthias KloorMagnus von Knebel Doeberitz
Aug 10, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Heather W Stout-DelgadoDwain L Thiele
Dec 2, 2014·International Ophthalmology Clinics·Maria M ChoudharyArun D Singh
Nov 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heather W Stout-DelgadoDwain L Thiele
Apr 15, 2015·Oncotarget·Ido Didi FabianOsnat Ashur-Fabian
Jan 24, 2019·Frontiers in Endocrinology·Osnat Ashur-FabianIdo Didi Fabian
Dec 16, 2020·Cancers·Asad Javed, Mohammed Milhem

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis