Interferon-alpha-2b downregulation of oncogenes H-ras, c-raf-2, c-kit, c-myc, c-myb and c-fos in ESKOL, a hairy cell leukemic line, results in temporal perturbation of signal transduction cascade

Leukemia Research
William HarveyN A Heerema

Abstract

ESKOL, a B-lymphoblastoid cell line consisting of late differentiated cells, resembles hairy cell leukemia (HCL). It is pseudodiploid with a deleted 7q and an unbalanced translocation between chromosomes 4 and 6. It was screened by Northern hybridization for oncogenes, including H-ras, c-raf-2 (c-raf1p1), c-kit, c-myc, c-myb, c-fos, Fim-1, c-jun, ski, and c-mos, which are believed to contribute to B-cell differentiation and maturation. Interferon-alpha-2b (IFN) downregulates the expression of H-ras, c-raf-2, c-kit, c-myc, c-myb, c-fos, as determined by Northern hybridization of RNA isolated from cells harvested at time points during a 30 h time course. Downregulation of oncogenes H-ras, c-raf-2, c-kit, whose proteins are associated with cell surfaces or are cytosolar transducers, occurs before those oncogenes c-myc, c-myb, and c-fos, whose products are DNA binding proteins. This suggests a temporal perturbation of signal transduction by IFN. No change in oncogene expression occurred in non-treated cells nor were these oncogenes expressed in the non-transformed B-lymphoblast cell line, Wil-2, under the same treatment regimen. The basis for the IFN perturbation is not understood; yet the role of these oncogenes as signal transduc...Continue Reading

References

Dec 1, 1990·The Journal of Experimental Medicine·H E HeslopM K Brenner
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J B GibbsE M Scolnick
Mar 1, 1988·European Journal of Biochemistry·D H Wreschner, G Rechavi
Oct 30, 1971·Lancet·M Seabright
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D DiMaioT Maniatis
Mar 15, 1984·Biochemical and Biophysical Research Communications·L E Grosso, H C Pitot
Jun 1, 1981·Human Pathology·J J Yunis
Jun 1, 1993·Behavioral Neuroscience·R GlaserJ K Kiecolt-Glaser
May 1, 1993·Molecular and Cellular Biology·J P CogswellJ P Ting

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Citations

Oct 24, 2002·Tissue Antigens·L C Goldie-CreganL J Abraham
Nov 5, 2002·Nature Reviews. Cancer·Mark J SmythHideo Yagita
Mar 12, 2002·British Journal of Haematology·Ugo ConsoliRosario Giustolisi
Feb 5, 1998·British Journal of Haematology·C PreudhommeP Fenaux
Sep 1, 1999·British Journal of Haematology·H W AunerH Sill
Oct 16, 1999·British Journal of Haematology·J MiyauchiS Mizutani
Sep 21, 2000·British Journal of Haematology·S PoulainP Fenaux
Sep 1, 2001·British Journal of Haematology·E P Das-GuptaN H Russell
Jan 1, 1996·Vox Sanguinis·Y M Miller, H G Klein
Aug 22, 2003·The New England Journal of Medicine·Bob LöwenbergUNKNOWN Swiss Group for Clinical Cancer Research
Aug 1, 1995·Journal of Hematotherapy·H G Klingemann
Oct 1, 1995·British Journal of Haematology·L M Secker-WalkerB Bain
Sep 10, 2015·Journal of Translational Medicine·Junjie ChenChengshui Chen
Sep 14, 2007·Growth Factors·Sibel Berker KarauzumOsman Nidai Ozes
Jun 19, 2008·Cancer Research·Sylvie Galiègue-ZouitinaCarl Simon Shelley

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