PMID: 8944670Nov 1, 1996Paper

Interferon-alpha downregulates expression of the oxytocin receptor in cultured human myometrial cells

The American Journal of Physiology
M MaggiM Serio

Abstract

Previous studies in the endometrium of ruminants showed that type I interferon (IFN) prevents oxytocin receptor (OTR) formation. We studied the effect of IFN-alpha on human myometrial cells in culture expressing a high density of biologically active OTR. We found that IFN-alpha induced a 35-50% decrease in OTR mRNA and protein and that this inhibition was time and dose dependent. Maximal inhibition of OTR mRNA was obtained after 2-3 days, whereas 1-(beta-mercapto-beta, beta-cyclopentamethyl-enepropionic acid,2-O-Me-Tyr,Thr4,Orn8,Tyr9-amide)-[125I]vasotocin ([125I]OTA) binding reached a nadir after 3-4 days, with half-maximal inhibitory concentration (IC50) = 1,100 U/ml. Mathematical analysis of multiple homologous competition curves for [125I]OTA indicated that IFN-alpha treatment (5,000 U/ml x 3 days) reduced just the binding capacity (Bmax) without changing the binding affinity. Accordingly, the same treatment with IFN-alpha did not affect the half-maximally effective concentration (EC50) for the oxytocin-induced increase in intracellular calcium but significantly decreased maximal responsiveness (Emax) of myometrial cells to OT stimulation. In conclusion, our data demonstrate, for the first time, a negative regulation by IFN...Continue Reading

References

Jul 8, 1975·Biochemical and Biophysical Research Communications·M S Soloff
Jun 22, 1979·Science·M S SoloffM J Fernstrom
Apr 9, 1992·Nature·T KimuraH Okayama
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·M MaggiM Serio
Apr 1, 1990·The Journal of Clinical Endocrinology and Metabolism·M MaggiM Serio
Jun 1, 1986·Journal of Interferon Research·A A Branca
Jan 1, 1985·The Journal of Clinical Endocrinology and Metabolism·A R FuchsM S Soloff
Mar 1, 1986·Journal of Reproduction and Fertility·F W BazerW W Thatcher
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·F RozenH H Zingg
Oct 1, 1994·Journal of Endocrinological Investigation·M MaggiT Susini
May 28, 1994·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·A P FlintD R Abayasekara
Feb 1, 1994·American Journal of Obstetrics and Gynecology·T M GoodwinR Merriman
Mar 1, 1994·Biology of Reproduction·J S Hunt
Nov 1, 1995·British Journal of Obstetrics and Gynaecology·T BossmarM Maggi

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Sep 27, 2018·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·C DingF Magkos

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