Interferon-alpha inhibits cyclooxygenase-1 and stimulates cyclooxygenase-2 expression in bladder cancer cells in vitro

Urological Research
P J BoströmM Laato

Abstract

The enzymes cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) catalyze the initial step in the formation of prostaglandins (PGs). PGs are known to be involved in numerous processes, for example inflammation, immune responses, carcinogenesis, and tumor angiogenesis. The formation of PGs is stimulated in various cancers since the expression of Cox-2 is upregulated. Interferon (IFN)-alpha is used in the treatment of bladder cancer, although not all of the effects of such treatment are thoroughly known. Therefore, we investigated the expression of cyclooxygenases in two bladder cancer cell lines, 5637 and T24, under basal conditions and in the presence of human recombinant IFN-alpha (100, 1,000, and 10,000 U/ml). The mRNA of Cox-1 and Cox-2 was expressed in both cultured bladder carcinoma cell lines. The level of Cox-1 expression was low in 5637 cells and higher in T24 cells. In contrast, Cox-2 expression was prominent in 5637 cells and low in T24 cancer cells. The highest IFN-alpha concentration (10,000 U/ml) decreased the expression of Cox-1 to 47 and 28% of the control levels in 5637 and T24 cells, respectively. In contrast, Cox-2 expression increased in both cell lines. In 5,637 cells, Cox-2 expression increased 1.3-fold wi...Continue Reading

Citations

Oct 2, 2002·Current Oncology Reports·Daniel J Lindner
Jun 23, 2009·Cancer Biotherapy & Radiopharmaceuticals·Nuria AlvarezCristina Martínez
Aug 7, 2003·American Journal of Clinical Oncology·Asad UmarErnest T Hawk
Jul 1, 2008·Expert Review of Anticancer Therapy·John A TaylorAndrew Nisbet
Jan 1, 2005·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Dionisios MitropoulosVassilis G Gorgoulis
Aug 25, 2019·Medical Hypotheses·Ersoy Öksüz, Muhammet Serdar Buğday

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