PMID: 8943096Dec 1, 1996Paper

Interferon-beta inhibits proliferation and progression through S phase of the cell cycle in five glioma cell lines

Journal of Neuro-oncology
J I GarrisonG Floyd-Smith

Abstract

The growth inhibitory effect of IFN-beta was evaluated in 5 human glioma cell lines (AO2V4, GJC, GJR, NN and NNR) and in normal astrocyte cultures (SC and TM). All 5 glioma cell lines showed an anti-proliferative response to IFN-beta whereas normal glial cells were non-responsive. IFN-beta at 10, 100 and 500 U/ml lead to a 30%, 70% and 80% relative decrease in cell number after 12 days, respectively in AO2V4 cells. GJC and GJR cell lines also responded significantly to the lowest concentration of IFN-beta tested and at 500 U/ml the relative cell number decreased 55%. The NN and NNR cells were the least responsive to IFN-beta with maximum growth inhibition of 30% at 500 U IFN-beta/ml. Following treatment with IFN-beta, AO2V4, GJC, GJR and normal astrocytes all expressed mRNA encoding the anti-viral protein, 2-5A synthetase demonstrating that IFN-beta bound to receptors on all four cell lines and activated signal transduction pathways required for induction of an anti-viral protein. A determination of the relative number of viable cells showed that none of these cells exhibited a significant decrease in cell viability. Since the antiproliferative response to IFN-beta was not primarily due to cell death, the effect of IFN-beta on ...Continue Reading

Citations

Jun 12, 2003·Journal of Neuroimmunology·Olga BarcaVíctor M Arce
Jul 22, 1998·Journal of Neuroimmunology·W RothM Weller
Mar 26, 2003·International Journal of Radiation Oncology, Biology, Physics·Heinz SchmidbergerC F Hess
Jul 1, 2009·Molecular and Cellular Biology·Ignacio MoragaSandra Pellegrini
Oct 13, 2004·Neuroendocrinology·Stefan RosewiczZ von Marschall
Aug 19, 2007·Neuroimmunomodulation·Olga BarcaVíctor M Arce
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Sep 15, 2018·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Jae-Rim HeoKyung-Chul Choi
Apr 24, 2020·Journal of Cellular Physiology·Mehnoosh Ashja-ArvanMazdak Ganjalikhani-Hakemi
Jun 18, 2020·International Journal of Molecular Sciences·Kim M A De Kleijn, Gerard J M Martens
Nov 20, 2018·Frontiers in Immunology·Elisabeth MüllerAlexandre Corthay
Nov 23, 2021·Frontiers in Physiology·Hyun Jun JungPaul A Welling

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