PMID: 4352965Jul 1, 1973

Interferon: evidence for its glycoprotein nature

Proceedings of the National Academy of Sciences of the United States of America
F DornerR Weil

Abstract

In an attempt to understand the structure of rabbit interferon, the possibility of carbohydrate being part of the molecule was tested. Interferon incubated with neuraminidase from Vibrio cholera is homogeneous in charge as revealed by isoelectric focusing. Treatment of "asialointerferon" with galactose oxidase (EC 1.1.3.9) from Dactylium dendroides and subsequent reduction with tritiated sodium borohydride yields labeled material with unimpaired antiviral activity. Enzymic incorporation of N-[(14)C]acetylneuraminic acid into tritiated asialointerferon restores the original charge heterogeneity. The newly generated sialointerferon contains both (3)H and (14)C activity. Asialointerferon is retained by an affinity column containing phytohemagglutinin from Phaseolus vulgaris and can be displaced from the adsorbent by a glycoprotein of known structure. It is concluded that rabbit interferon is a glycoprotein containing the terminal oligosaccharide sequence sialic acid --> galactose.

References

Oct 30, 1969·The New England Journal of Medicine·R G Spiro
Aug 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·S Kornfeld, R Kornfeld
Sep 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·Y H TanM Ho
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Citations

Aug 1, 1978·Biulleten' eksperimental'noĭ biologii i meditsiny·I Z ZaretskiĭF I Ershov
Jan 1, 1984·Archives of Virology·E A Havell, G L Spitalny
Jan 1, 1980·Archives of Virology·K C ChadhaE Sulkowski
Jan 1, 1981·Pharmacology & Therapeutics·V Bocci
Aug 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C B AnfinsenD Gurari-Rotman
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J W McCray, R Weil
Jan 1, 1974·Preparative Biochemistry·W E StewartE De Clercq
Jan 1, 1979·Preparative Biochemistry·E Bollin, E Sulkowski
Jan 1, 1980·Preparative Biochemistry·A J MikulskiE Sulkowski
Jul 1, 1982·The Journal of Experimental Medicine·E A HavellP J Patel
Jan 1, 1988·Journal of Veterinary Internal Medicine·K L Jacobsen, G A Rockwood
Oct 16, 1978·Biochemical and Biophysical Research Communications·Y IwakuraY Kawade
Jun 1, 1978·Analytical Biochemistry·D Malamud, J W Drysdale
Aug 30, 1974·Nature·F Besancon, H Ankel
Jun 1, 1974·Acta Pathologica Et Microbiologica Scandinavica. Section B: Microbiology and Immunology·K E MogensenK Cantell

Related Concepts

Carbon Isotopes
Chromatography, Affinity
Neoglycoproteins
Interferons
Isoelectric Focusing
Kidney
Neuraminic Acids
Neuraminidase
Bacteriophage Plaque Assay
Tritium

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