PMID: 2110799May 15, 1990Paper

Interferon-gamma alters expression of endothelial cell-surface glycosphingolipids

Archives of Biochemistry and Biophysics
B K GillardD M Marcus

Abstract

Our previous work on human endothelial cell (EC) glycosphingolipids (GSLs) demonstrated that these cells contain a large diversity of GSLs, predominantly with lacto core structures. In order to evaluate the role of GSLs as EC antigens and receptors, we investigated their cell-surface expression on confluent EC monolayers and ECs activated by interferon-gamma (IFN-gamma) and interleukin-1 (IL-1). IFN-gamma activation of endothelial cells resulted in a small change in GSL composition, but greatly increased surface expression of gangliosides and decreased surface expression of neutral GSLs. In particular, surface expression of the major neutral GSL, globoside, decreased three- to fourfold as measured both by galactose oxidase labeling and by binding of the anti-globoside monoclonal antibody 9G7. IFN-gamma did not significantly alter the total cell content of globoside, as measured by metabolic labeling, but rather altered the ratio of accessible cell surface to intracellular globoside. Two mechanisms appear to contribute to the decreased cell-surface globoside expression. IFN-gamma treatment increased the relative proportion of intracellular globoside which is associated with the cell cytoskeleton. IFN-gamma treatment also caused ...Continue Reading

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Citations

Feb 16, 1996·Biochimica Et Biophysica Acta·A AbeJ A Shayman
Jan 1, 1994·Progress in Biophysics and Molecular Biology·B Maggio
Aug 26, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·E W Holmes
Jun 14, 2013·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Donald M Marcus
Oct 31, 2013·International Journal of Vascular Medicine·Norihiko Sasaki, Masashi Toyoda
Jul 7, 2012·Cellular and Molecular Life Sciences : CMLS·Andreas BauwensJohannes Müthing
Dec 2, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N KovacicA Marusic
Feb 15, 2005·Biochimica Et Biophysica Acta·Roger SandhoffHermann-Josef Gröne

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