Interferon gamma and calcitonin induce differential changes in cellular kinetics and morphology of osteoclasts in cultured neonatal mouse calvaria

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
K KlaushoferM Peterlik

Abstract

The present study compares the effects of calcitonin (CT) and interferon gamma (IFN-gamma) on the size, distribution, and ultrastructure of osteoclasts in cultured neonatal mouse calvaria. The number and cross-sectional area of osteoclasts in cultured bones was increased by the addition of parathyroid hormone (PTH) to the culture medium for 24-48 h. Prolonged treatment (up to 72 h) with PTH led to extensive rarefication and formation of holes in the mineralized matrix. PTH-activated osteoclasts exhibited an elaborate ruffled border and showed a typical zonal arrangement of intracellular organelles with the outer cytoplasmic region containing numerous membrane-bound vesicles. CT (20 mU/ml) within 90 minutes caused a complete loss of the ruffled border in PTH-activated osteoclasts. The typical zonal architecture disappeared with intracellular vesicles spread through the entire cytoplasm. Prolongation of CT treatment to 24-48 h led to the appearance of vesicles with dark granular content in inactivated osteoclasts. This morphologically distinct vesicle population allowed us to identify the latter cells as "postosteoclasts" and thereby to distinguish them from osteoclasts precursors. Small active osteoclasts that had originated ver...Continue Reading

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Citations

Jan 1, 1991·Electron Microscopy Reviews·A M PierceL Hammarström
Jul 1, 1997·Experimental Gerontology·M Peterlik
Apr 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M IkegameD M Findlay
Jun 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S YumitaT J Martin
Jan 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M IkegameH Ozawa
Mar 12, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·J T NinomiyaE M Greenfield
Jun 1, 1996·Oral Diseases·S H WiebeS J Dixon
Sep 1, 1996·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·I SembaY Miura
Mar 13, 1999·Archives of Oral Biology·N Kawashima, P Stashenko

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