Interferon-gamma-dependent nuclear import of Stat1 is mediated by the GTPase activity of Ran/TC4.

The Journal of Biological Chemistry
T SekimotoY Yoneda

Abstract

In response to interferon-gamma (IFN-gamma), Stat1 enters the nucleus, where it activates transcription. In order to better understand the mechanism of the extracellular signal-induced protein import into the nucleus, we have established an in vivo assay system that uses recombinant Stat1 protein as a model transport substrate. Using this system, we found that Stat1 is actively transported through the nuclear pores in an IFN-gamma-dependent manner and tyrosine (Tyr701) phosphorylation of Stat1 is actually required for its nuclear import. When the antibody against Ran, which was identified as an essential factor for active nuclear protein transport, was injected, the IFN-gamma-dependent nuclear transport of Stat1 was completely inhibited. Furthermore, nuclear import of Stat1 was suppressed by microinjection of two mutant Ran proteins, one defective in GTP hydrolysis (G19V) and the other with little or no binding to GTP (T24N), both of which are known to act as dominant negative inhibitors of nuclear import. These results indicate that the conditional nuclear import of Stat1 requires GTP hydrolysis by Ran.

References

Jun 1, 1990·The Journal of Cell Biology·C M StarrJ A Hanover
Feb 1, 1987·The Journal of Cell Biology·D R FinlayD J Forbes
Jul 1, 1995·The Journal of Cell Biology·N C ChiS A Adam
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·G SchlenstedtP A Silver
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A RaduM S Moore
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·J MoroianuA Radu
May 1, 1994·Trends in Biochemical Sciences·J N IhleO Silvennoinen
Jan 1, 1995·Methods in Enzymology·F MelchiorL Gerace
May 1, 1996·The Journal of Cell Biology·I PalaciosC Dingwall

❮ Previous
Next ❯

Citations

Jul 19, 2012·In Vitro Cellular & Developmental Biology. Animal·Jian-Guo HuangXin Jin
Oct 11, 2003·Pharmacology & Therapeutics·Dhananjaya V Kalvakolanu
Jan 26, 1999·Cytokine & Growth Factor Reviews·T Sekimoto, Y Yoneda
Jun 10, 1998·Cytokine & Growth Factor Reviews·T HiranoM Hibi
Mar 28, 2008·Cell Research·Jinbo Yang, George R Stark
May 24, 2005·Nature Immunology·Kim A BrogdenMichael Zasloff
Jul 27, 2006·Nature Reviews. Immunology·Nancy C Reich, Ling Liu
Oct 6, 2011·Nature Reviews. Urology·Vincent GoffinMarja T Nevalainen
Jul 7, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J LarkinH M Johnson
Apr 10, 1999·Molecular Biology of the Cell·F YokoyaY Yoneda
Dec 18, 2009·Molecular Biology of the Cell·Yutaka OgawaYoshihiro Yoneda
Dec 21, 2004·European Journal of Biochemistry·Thomas Meyer, Uwe Vinkemeier
Sep 13, 2000·Molecular and Cellular Biology·K Mowen, M David
Oct 23, 2008·PloS One·Shuo WangAntonis E Koromilas
Oct 20, 1999·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·N Yamamoto, X W Deng
Sep 12, 2000·Current Opinion in Genetics & Development·J G Williams
Oct 27, 2004·The Journal of Cell Biology·Uwe Vinkemeier
Feb 18, 2003·The Journal of Clinical Investigation·Samita Bhattacharya, Christian Schindler
Oct 6, 1998·Annual Review of Biochemistry·G R StarkR D Schreiber
Aug 14, 2003·Science's STKE : Signal Transduction Knowledge Environment·Kevin M McBride, Nancy C Reich
Jun 13, 2000·Oncogene·C V RamanaG R Stark
Jun 13, 2000·Oncogene·J HerringtonC Carter-Su
Sep 8, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Howard M JohnsonDavid A Jans
Sep 6, 2008·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Andreas MargUwe Vinkemeier
Apr 20, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hideki YamasakiYoshihiro Yoneda
Jan 5, 2000·The Journal of Biological Chemistry·C L YuS J Burakoff
Dec 29, 1998·The Journal of Biological Chemistry·P S SubramaniamH M Johnson
Nov 7, 1998·The Journal of Biological Chemistry·K Mowen, M David
Jul 31, 1999·The Journal of Biological Chemistry·A V KazanskyJ M Rosen
Aug 28, 1999·The Journal of Biological Chemistry·M I NdubuisiP B Sehgal

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.