Interferon-gamma induces a decrease in the intracellular calcium pump in a human salivary gland cell line

The American Journal of Physiology
S MeehanI S Ambudkar

Abstract

Interferon-gamma (IFN-gamma) +/- tumor necrosis factor-alpha (TNF-alpha) induces antiproliferation and intracellular Ca2+ store depletion in a human submandibular ductal cell line (HSG), which can be reversed on cytokine removal [A. J. Wu, G. C. Chen, B. J. Baum, and I. S. Ambudkar. Am. J. Physiol. 270 (Cell Physiol. 39): C514-C521, 1996]. Here we have examined a possible mechanism for the IFN-gamma-induced intracellular Ca2+ store depletion. There was a time-dependent decrease in thapsigargin-dependent internal Ca2+ release after exposure of the cells to the cytokines. The intracellular Ca2+ pump [sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA)] protein in lysates and membranes of cells treated with IFN-gamma +/- TNF-alpha, but not with TNF-alpha alone, showed a similar time-dependent decrease (examined using a SERCA2 antibody). Removal of the cytokines, which resulted in recovery of cell growth and refill of internal Ca2+ stores, also increased the level of SERCA protein. The decrease in SERCA is not a result of decreased cell proliferation, since thapsigargin, 2,5-di-(t-butyl)-1,4-hydroquinone, or serum-free growth conditions induced antiproliferative effects on HSG cells without any corresponding decrease in SERCA. We su...Continue Reading

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Citations

Feb 22, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·I S Ambudkar
Mar 14, 2013·American Journal of Reproductive Immunology : AJRI·Gabriela CarrasquelMarie C Ruiz
May 4, 2016·Proceedings of the National Academy of Sciences of the United States of America·Zhennan LaiJohn A Chiorini
Feb 15, 2001·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S K PiechnikJ D Pickard
Nov 5, 2005·Histochemistry and Cell Biology·Veronika HomannRolf K-H Kinne
Mar 22, 2012·Journal of Vascular Research·Hai-Chao Han
Oct 31, 2001·Archives of Oral Biology·X LiuG H Zhang
Oct 6, 2009·Journal of Autoimmunity·J A ChioriniP Caturegli

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