Interferon-gamma-inducible protein p16. A new target of antinuclear antibodies in patients with systemic lupus erythematosus

Arthritis and Rheumatism
H P SeeligM Renz

Abstract

To determine the cellular expression and localization of interferon-gamma (IFN gamma)-inducible protein p16, a new antigen specificity of antinuclear antibodies (ANA), and to evaluate the prevalence of anti-p16, particularly in SLE patients. Serum levels of anti-p16 were determined by immunoblotting with recombinant p16 and cellular p16 messenger RNA (mRNA) by Northern blotting. We also utilized immunoprecipitation of 35S-methionine-labeled proteins, immunostaining of blotted proteins of subcellular fractions, and immunofluorescence studies with affinity-purified rabbit and human anti-recombinant p16. Protein p16 was localized within the nucleolus and nucleoplasm and constitutively expressed in Raji, HeLa, HEp-2, K562, and HL-60 cells. Synthesis of mRNA and protein was increased in the presence of IFN gamma. The prevalence of anti-p16 was 29% in 374 SLE patients (35% in those positive for anti-double-stranded DNA) and 0% in 188 healthy individuals. Anti-p16 was always accompanied by positive findings on indirect immunofluorescence for ANA. Anti-p16 represents a main ANA specificity. Anti-p16 may help to elucidate IFN gamma-dependent nuclear processes and to link autoantibody production to states of IFN gamma activation.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 20, 1991·Journal of Immunological Methods·H P SeeligM Renz
Jul 1, 1990·Clinical and Experimental Immunology·C GelpiJ L Rodriguez-Sanchez
Jan 1, 1987·The Journal of Clinical Investigation·G ReimerE M Tan
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H A GoldS Altman
May 5, 1989·Journal of Molecular Biology·M Suzuki
Dec 1, 1986·Annals of the Rheumatic Diseases·J PfeifleM Franke
May 14, 1987·Biochemical and Biophysical Research Communications·D A StetlerS T Jacob
Jun 1, 1969·Arthritis and Rheumatism·N WatanabeW V Epstein
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein
Jan 1, 1984·Journal of Clinical Immunology·M ReichlinE M Tan
Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester
Apr 1, 1994·Molecular and Cellular Biology·H P SeeligM Renz
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander

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Citations

Jul 12, 2002·Journal of Cellular Biochemistry·Kevin L DoggettRobert C Briggs
May 18, 2005·Experimental Cell Research·Louise E A LudlowChristopher J P Clarke
Dec 29, 1998·Biochimie·S LandolfoD Lembo
Jan 5, 2010·Nature Reviews. Rheumatology·John C Hall, Antony Rosen
Jan 5, 2011·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Marisa GariglioSanto Landolfo
Oct 6, 2007·Current Opinion in Rheumatology·Jennifer G Walker, Marvin J Fritzler
Jun 21, 2014·Immunological Investigations·Ronni Rômulo Novaes E BritoMario Mariano
Dec 14, 2007·Autoimmunity·Bahram NamjouJohn B Harley
Oct 4, 2007·Annals of the New York Academy of Sciences·Michele MondiniSanto Landolfo
Apr 17, 2015·Immunological Reviews·Tsan Sam Xiao
Sep 7, 2014·Cytokine & Growth Factor Reviews·Siobhán Smith, Caroline Jefferies
Jan 30, 2013·Journal of Immunological Methods·Alessandra DellavanceLuis Eduardo Coelho Andrade
Oct 12, 2004·Arthritis and Rheumatism·Wolfgang HueberPaul J Utz
Jul 3, 2010·The Journal of Rheumatology·Ingorn KimkongNattiya Hirankarn
Aug 19, 2007·The Journal of Biological Chemistry·Patrizia CaposioSanto Landolfo
May 10, 2016·Arthritis & Rheumatology·Zsuzsanna H McMahanLivia Casciola-Rosen
Jan 18, 2018·International Journal of Rheumatic Diseases·Rongbin LiJianwei Li
Nov 9, 2018·Frontiers in Immunology·Sonya J WolfJ Michelle Kahlenberg
Feb 7, 2004·The Journal of Biological Chemistry·Rocky PramanikDivaker Choubey
Oct 27, 2004·Seminars in Arthritis and Rheumatism·Yaniv ShererYehuda Shoenfeld
May 24, 2008·Immunology Letters·Luiz Felipe Leomil CoelhoErna Geessien Kroon

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