Interferon gamma is recognised by importin alpha/beta: enhanced nuclear localising and transactivation activities of an interferon gamma mimetic.

FEBS Letters
Alex James FulcherDavid A Jans

Abstract

Interferon (IFN) gamma's ability to localise in the nucleus and function in gene activation has been known for some time, although the role of the conventional nuclear transporting importin molecules is unclear. Here, we demonstrate for the first time the direct recognition of IFNgamma and an IFNgamma mimetic peptide by IMPalpha and the IMPalpha/beta heterodimer, where the IFNgamma mimetic shows higher affinity. Significantly, this correlates well both with in vivo ability to target green fluorescent protein to the nucleus in transfected cells as determined by quantitative confocal laser scanning microscopy, as well as GAS promoter activity of a luciferase reporter. This has important implications for IFNgamma's anti-viral action, and the potential use of the IFNgamma mimetic in antiviral therapies.

References

Jul 16, 1986·Biochemical and Biophysical Research Communications·H S MacDonaldS E Grossberg
Sep 30, 1994·Biochemical and Biophysical Research Communications·B E SzenteH W Johnson
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·T Bader, J Weitzerbin
Oct 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·B E SzenteH M Johnson
Dec 29, 1998·The Journal of Biological Chemistry·P S SubramaniamH M Johnson
Dec 19, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·P S SubramaniamH M Johnson
May 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vincent R HarleyDavid A Jans
Jun 12, 2003·Journal of Cell Science·C M Iqbal AhmedHoward M Johnson
May 5, 2004·Biochemistry·Prem S SubramaniamHoward M Johnson
Sep 8, 2004·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Howard M JohnsonDavid A Jans
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chulbul M I Ahmed, Howard M Johnson
Nov 7, 2006·Trends in Cell Biology·Bryan Linggi, Graham Carpenter
Aug 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Becky L Conway-CampbellMichael J Waters

❮ Previous
Next ❯

Citations

Jun 15, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chulbul M I AhmedHoward M Johnson
Jan 13, 2017·Mediators of Inflammation·Howard M Johnson, Chulbul M Ahmed
May 13, 2009·Current Opinion in Lipidology

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.