PMID: 8440906Mar 1, 1993Paper

Interferon gamma regulation of de novo protein synthesis in human dermal fibroblasts in culture is anatomic site dependent

The Journal of Investigative Dermatology
T J Smith, P J Higgins

Abstract

The propensity of the skin of the lower anterior leg to be involved in Graves' dermopathy prompted an examination of the specific protein synthesis and response to interferon gamma in cultured fibroblasts from this area. Confluent cultures from normal skin of the lower leg and from the abdomen of the same three donors were pulse labeled with [35S]methionine for 3 h and subjected to two-dimensional protein gel electrophoresis and fluorography. Protein spots were mapped using a computer-driven program and the relative densities of the resolvable spots analyzed. Fibroblasts from the two anatomic sites display distinct patterns of de novo protein synthesis. Of the 157 abundant spots arbitrarily chosen for analysis, 31% varied substantially in levels of expression between the sites. A number of proteins appear to be expressed only in cultures derived from one of the two anatomic sites. Interferon gamma (100 U/ml) present in the culture medium for 48 h influenced the abundance of a number of proteins in a site-specific manner. Among them, plasminogen activator inhibitor type-1 was induced three to five times in the leg cultures, whereas this same polypeptide was down-regulated in abdominal fibroblasts. A 54-kD protein was induced in ...Continue Reading

References

Jul 1, 1978·The Journal of Investigative Dermatology·H S CheungM E Nimni
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·T M HassellC G Cooper
Jul 1, 1992·The American Journal of Physiology·T J SmithP J Higgins
May 1, 1991·The Journal of Clinical Endocrinology and Metabolism·T J SmithM Cheavens
Aug 1, 1989·Endocrine Reviews·T J SmithC A Gorman
Jan 31, 1972·Biochemical and Biophysical Research Communications·L PinskyG Hall
Apr 1, 1984·Journal of Cutaneous Pathology·M IshiiT Hamada
Nov 1, 1982·The Journal of Clinical Investigation·T J SmithS Refetoff
Apr 1, 1980·Journal of Cutaneous Pathology·K KonradH Pehamberger

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Citations

Dec 1, 1994·Cell and Tissue Research·R C Henrikson, T J Smith
Jul 1, 1996·International Journal of Dermatology·M M al-Khawajah
Mar 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D SciakyT J Smith
Oct 30, 2016·The Biochemical Journal·José Antonio Hernández-TrejoPorfirio Nava
Jan 1, 1994·Nature Genetics·J G Compton
Jul 28, 2005·Clinical and Experimental Immunology·T J Smith
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·D A YoungT J Smith
Jul 1, 1994·The British Journal of Dermatology·G C PriestleyD Wilson
Feb 21, 2021·Advances in Wound Care·Meilang XueChristopher Jackson
Aug 1, 1995·The American Journal of Physiology·M G HoggT J Smith
Jul 1, 1993·The American Journal of Physiology·T J SmithT T Andersen
Jul 22, 1999·Archives of Oral Biology·A M CornelissenA M Kuijpers-Jagtman

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