PMID: 7539429Jun 2, 1995Paper

Interferon-induced MxA protein. GTP binding and GTP hydrolysis properties.

The Journal of Biological Chemistry
M F RichterP Staeheli

Abstract

MxA is a GTPase encoded by an interferon-activated human gene which inhibits the multiplication of several RNA viruses. Recombinant histidine-tagged MxA protein (His-MxA) was expressed in Escherichia coli and purified to near homogeneity. Gel filtration showed that it formed high molecular weight oligomers. Purified His-MxA exhibited specific GTP hydrolysis rates of up to 350 nmol of GTP/min/mg of protein, corresponding to a turnover number of 27 min-1. The Km for this reaction was 260 microM. Guanine nucleotides did not copurify with His-MxA. Binding experiments in solution with fluorescent-labeled nucleotides confirmed that His-MxA binds guanine nucleotides rather weakly and further showed that the fluorescent GDP analog N-methylanthraniloyl (mant)-GDP had a much lower affinity for His-MxA (Kd 20 microM, koff 8.5 s-1) than the nonhydrolyzable GTP analog mant-5'-guanylyl-beta,gamma-imidotriphosphate (mant-GMP-PNP) (Kd 0.75 microM, koff 0.012 s-1). Competitive binding studies with nonlabeled nucleotides revealed a similar binding preference of His-MxA for GTP over GDP: the Kd for GTP was 20 microM, whereas the Kd for GDP was 100 microM. Thus, a high percentage of MxA molecules may be complexed with GTP in vivo.

References

Jan 30, 1992·Nature·J E Rothman, L Orci
Oct 1, 1991·Trends in Biochemical Sciences·A Wittinghofer, E F Pai
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J S HerskovitsR B Vallee
May 1, 1994·Trends in Biochemical Sciences·M S Moore, G Blobel
Apr 1, 1994·Trends in Biochemical Sciences·G Fischer von MollardR Jahn
Jun 1, 1994·Trends in Biochemical Sciences·M Sprinzl
Aug 1, 1991·Trends in Cell Biology·C A Collins
Apr 1, 1993·Current Biology : CB·P Melançon
Nov 1, 1993·Current Biology : CB·I S Trowbridge

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Citations

Jun 27, 2000·Virus Research·M MarschallO Haller
Apr 14, 1999·Trends in Cell Biology·A M van der Bliek
Sep 9, 2011·The Journal of Biological Chemistry·Alexander von der MalsburgDganit Danino
Jun 12, 2010·The Journal of Biological Chemistry·Otto HallerGeorg Kochs
Aug 24, 2000·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·V Jensen, B Robertsen
Feb 24, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M PalmD Desmecht
Jun 12, 2009·Molecular Biology of the Cell·Joshua S ChappieSandra L Schmid
Jan 10, 1998·The EMBO Journal·A B MuhlbergS L Schmid
Jan 23, 1999·Immunological Reviews·J C LeongB Simon
Aug 10, 2000·Journal of Virology·C JanzenO Haller
Oct 6, 1998·Annual Review of Biochemistry·G R StarkR D Schreiber
Oct 14, 2000·Annual Review of Cell and Developmental Biology·J E Hinshaw
Jul 11, 2006·Annual Review of Cell and Developmental Biology·Sascha Martens, Jonathan Howard
Nov 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Thomas C MarlovitsVinzenz M Unger
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·G Kochs, O Haller
Sep 14, 2018·Reviews in Medical Virology·Olga V Matveeva, Peter M Chumakov
Jun 3, 2011·Journal of Virology·Benjamin SchusserSonja Härtle
Dec 4, 2013·Microbiology and Molecular Biology Reviews : MMBR·Judith VerhelstXavier Saelens
Mar 26, 2004·Nature Reviews. Molecular Cell Biology·Gerrit J K Praefcke, Harvey T McMahon
Feb 6, 1999·The Journal of Biological Chemistry·G Kochs, O Haller
Feb 16, 2002·The Journal of Biological Chemistry·Georg KochsOtto Haller
Jun 2, 1995·The Journal of Biological Chemistry·M SchwemmleP Staeheli
Oct 17, 1998·The Journal of Biological Chemistry·B Schumacher, P Staeheli

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