Interferon-induced protein IFIT4 is associated with systemic lupus erythematosus and promotes differentiation of monocytes into dendritic cell-like cells.

Arthritis Research & Therapy
Xiangyang HuangShunle Chen

Abstract

Using oligonucleotide microarray, many IFN-inducible genes have been found to be highly expressed in peripheral blood mononuclear cells (PBMCs) from most patients with systemic lupus erythematosus (SLE). Among these IFN-inducible genes, IFN-induced protein with tetratricopeptide repeats 4 (IFIT4) is a novel gene whose function is unknown. In this study we examined the role played by IFIT4 in monocyte differentiation and the correlation between IFIT4 expression and the clinical manifestation of SLE. To this end, we used plasmid transfection, flow cytometry, mixed leucocyte responses, ELISA, quantitative RT-PCR and Western blotting. We found that both IFIT4 mRNA and protein expression levels were significantly higher in PBMCs and monocytes from SLE patients than in those from healthy control individuals. IFIT4 expression was positively correlated with antinuclear antibodies, anti-double-stranded DNA, and anti-Sm auto-immune antibodies in SLE. Patients with SLE exhibiting higher expression of IFIT4 had a higher prevalence of leucopenia, thrombocytopenia and C3/C4 decrease. IFIT4 protein was localized exclusively to the cytoplasm, and it was significantly upregulated by IFN-alpha in normal PBMCs. To determine the role played by IFI...Continue Reading

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Citations

Oct 23, 2012·International Journal of Rheumatic Diseases·Ronald HerbstYihong Yao
May 25, 2012·Journal of Virology·Stephanie A SchittonePenny Clarke
May 21, 2010·PloS One·Jeannie L TeJoshua O Ojwang
Apr 2, 2016·Journal of Immunology Research·Xiaofeng LiaoXin M Luo
Feb 5, 2010·Arthritis and Rheumatism·Lisa G M van BaarsenCornelis L Verweij
Apr 11, 2017·Current Opinion in Rheumatology·Yahel SegalYehuda Shoenfeld
Sep 21, 2018·The Journal of General Virology·Harriet V Mears, Trevor R Sweeney
Jul 17, 2015·The Journal of Rheumatology·Estefanía Armas-GonzálezFederico Díaz-González
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Jan 17, 2009·Seminars in Arthritis and Rheumatism·Christina G KatsiariPetros P Sfikakis

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Methods Mentioned

BETA
density gradient separation
PCR
transfection
flow
ELISA
confocal microscopy
flow cytometry
fluorescence-activated cell sorting
FACS

Software Mentioned

ABI PRISM SDS

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