PMID: 6170316Aug 4, 1981Paper

Interferon induction by poly(inosinic acid).poly(cytidylic acid) segmented by spin-labels

Biochemistry
A M BobstE De Clercq

Abstract

Poly(inosinic acid).poly(cytidylic acid) [(I)n.(C)n] duplexes of which the (C)n strand was modified to various degrees chemically or enzymatically with nitroxide radicals (spin-labels) were evaluated for interferon-inducing activity. Upon annealing of the chemically modified (C)n, (1C,Cx)n (X = 1000 or 16), with (I)n, the interferon-inducing activity was similar to that of (I)n.(C)n in PRK cell cultures. However, to overcome hydrolysis of the spin-label linkage in (1C,Cx)n, and enzymatic approach was taken to synthesize (1S(4)U,Cx)n copolymers with x = 100, 38, 16, and 8. The (1s(4)U,Cx)n copolymers were chemically stable, and upon annealing with (I)n the correlation time of the nitroxide moiety in (I)n. (1s(4)U,Cx)n was determined. A comparison of this correlation time with that measured for (RUGT,U100)n.(A)n, which contains the nitroxide moiety in position 5 of the U moiety, suggests that the 1s(4)U residue is in a nonintrahelical conformation and partitions the duplex into double-helical segments of varying size. The interferon-inducing activity of (I)n. (1s(4)U,Cx)n was evaluated in primary rabbit kidney, human skin fibroblast (strain VGS), and mouse L-929 cell cultures as well as in rabbits. The 1s(4)U residue did not caus...Continue Reading

References

Aug 23, 1978·Biochimica Et Biophysica Acta·S KouidouA M Bobst
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A M BobstB Witkop
Feb 3, 1975·Biochemical and Biophysical Research Communications·P F TorrenceB Witkop
Jan 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·E De ClercqB Witkop
Apr 1, 1973·The Journal of General Virology·A BilliauP De Somer
Oct 14, 1972·Journal of Molecular Biology·W A CarterP O Ts'o
Apr 15, 1981·Archives of Biochemistry and Biophysics·P W Langemeier, A M Bobst

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