Interferon induction in marrow-derived macrophages: regulation by L cell conditioned medium

Journal of Cellular Physiology
H B Fleit, M Rabinovitch

Abstract

Mouse bone marrow cells grown in medium enriched with L cell conditioned medium (LCM) as a source of colony stimulating factor (CSF) yield populations of adherent macrophages which are quite sensitive to induction of interferon (IFN) by viral and nonviral inducers. We examined the role of LCM in the sensitivity of marrow macrophage cultures to IFN induction. Removal of LCM from the cultures for as little as 3 hours markedly reduced the IFN titers induced by a double stranded ribopolynucleotide (poly I:C) or a lipopolysaccharide (LPS), while induction by Newcastle disease virus (NDV) was unaffected. Addition of anti-CSF serum to LCM medium also reduced IFN titers in response to polyI:C but had no effect on NDV induction. The inhibitory effect of anit-CSF indicates that the LCM requirement is at least partially related to the colony stimulating activity of the medium. We postulate that CSF regulates the initial interaction of macrophages with polyI:C or LPS rather than the synthesis and secretion of interferon by the phagocytes. Nearly complete restoration of IFN induction with polyI:C was obtained when LCM deprived cultures were reincubated with LCM medium previously conditioned by marrow cultures.

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Citations

Sep 1, 1990·Journal of Neuroimmunology·P GalloB Tavolato
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D Metcalf, J G Moore
Jan 1, 1983·Journal of Interferon Research·H B Fleit
Jun 24, 2014·Journal of Cellular and Molecular Medicine·Sandra SalemPhilippe Gros
May 22, 1987·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D Metcalf
Jul 29, 1991·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D Metcalf
Feb 1, 1987·Immunology and Cell Biology·D Metcalf
Oct 1, 1992·European Journal of Immunology·K NohavaA Fontana
Apr 16, 2021·Life Science Alliance·Rachel E HeapMatthias Trost

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