Interferon induction in primates by stabilized polyriboinosinic acid-polyribocytidylic acid: effect of component size.

Infection and Immunity
H B LevyE L Stephen

Abstract

Two series of interferon-inducing complexes containing polyriboinosinic and polyribocytidylic acids, poly-L-lysine, and carboxymethyl cellulose were prepared. One series contained carboxymethyl cellulose, 27,000-molecular-weight poly-L-lysine, and either 4S, 6S, or 9S polyriboinosinic and polyribocytidylic acids. The other series contained carboxymethyl cellulose, 9S polyriboinosinic and polyribocytidylic acids, and poly-L-lysine, whose molecular weights ranged from 2,000 to 27,000. The homogeneity of these double-stranded polynucleotide complexes was confirmed by single-step thermal denaturation profiles and by single peaks in sucrose gradient velocity sedimentation. The complexes have a greater resistance to hydrolysis by ribonuclease than does polyriboinosinic-polyribocytidylic acid. The resistance to ribonuclease increased with the increasing size of polynucleotide homopolymers and poly-L-lysine. In monkeys and, to a lesser extent, in mice, serum interferon levels induced by the different complexes were related to the degree of resistance of the complexes to hydrolysis by ribonuclease. In mice, 4S, 6S, and 9S complexes of polyriboinosinic-polyribocytidylic acid, poly-L-lysine, and carboxymethyl cellulose had a higher level ...Continue Reading

References

Feb 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·J J NordlundH B Levy
Jan 1, 1966·Journal of Molecular Biology·M TsuboiP O Ts'o
Jul 1, 1959·Biochimica Et Biophysica Acta·G FELSENFELD, S HUANG

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Citations

Aug 8, 2015·PLoS Pathogens·Edward SionovKyung J Kwon-Chung
Jan 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·S K PuriR K Maheshwari
Jan 16, 2021·The Urologic Clinics of North America·Andres M Salazar, Esteban Celis

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