Interferon production by normal mouse tissues in organ cultures.

Journal of Interferon Research
I RosztóczyM Papós

Abstract

Freshly removed tissues of normal untreated mice produced relatively high amounts of interferon (IFN) in organ cultures. Lymph nodes, subcutaneous tissue, and the capsule of the kidney were the most active IFN producers. The abdominal wall and the thigh muscle were less active, whereas the lungs and spleen, similarly to the peritoneal exudate and bone marrow cells, produced only threshold amounts of IFN. Liver cultures did not produce IFN under these experimental conditions. Cultures prepared from IFN-pretreated animals produced three- to fourfold more IFN. Homogenates of tissue prepared immediately after their removal did not contain a detectable amount of IFN. The bulk of the IFN activity was produced during the first 6 h of incubation at 37 degrees C. Omission of serum from the culture medium, and the presence of 50 micrograms/ml of polymyxin B, did not inhibit IFN production. Cultures incubated at 0 degrees did not release any IFN. The IFN activity produced by all types of tissue was pH 2 resistant and it was neutralized by an antiserum to murine (Mu) IFN-beta. Different strains of mice produced comparable amounts of IFN under the present experimental conditions.

References

Dec 1, 1986·The Journal of General Virology·I Rosztóczy
Feb 1, 1981·Biological Reviews of the Cambridge Philosophical Society·V Bocci
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·F BelardelliI Gresser
Nov 1, 1982·European Journal of Immunology·J L Virelizier

❮ Previous
Next ❯

Citations

Jun 1, 1994·Immunology and Cell Biology·S Y HwangP J Hertzog

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.