PMID: 6161192Jan 1, 1980Paper

Interferon production in cocultures between mouse spleen cells and tumor cells: possible role of mycoplasmas in interferon induction

Journal of Immunological Methods
J BeckH Kirchner

Abstract

Interferon production was measured in the murine mixed lymphocyte tumor cell interaction (MLTI) using spleen cells of three inbred mouse strains and a number of in vitro grown lymphoma cells. No difference in interferon production was observed when syngeneic or allogeneic combinations were compared. Interferon was not detectable during the first 6 h of culture and reached its maximum after 12--24 h. In contrast, natural killer cell activity of spleen cells against one of these lymphoma lines (YAC-1) was already high after 4h. A cell line that was not susceptible to natural killing (MDAY-D2) induced the same amount of interferon as YAC-1. These findings suggest that natural killer cell activity and interferon induction in the MLTI are not correlated. Several lymphoma cell lines induced interferon in the MLTI whereas some others did not. All lines that were inductive were found to contain mycoplasmas. Furthermore, even the cell-free supernatants of inducing lines contained mycoplasmas and induced interferon. Purified mycoplasmas and membranes thereof were able to induce interferon production in mouse spleen cells. Our data strongly suggest that interferon production in the MLTI is caused by mycoplasmas. This artifact is the relev...Continue Reading

References

Jan 1, 1976·Infection·M Majer
Jan 1, 1978·Advances in Cancer Research·R B Herberman, H T Holden
Jul 1, 1976·Infection and Immunity·B C ColeL A Glasgow
May 20, 1976·Nature·G Biberfeld, E Gronowicz
May 1, 1972·Journal of Bacteriology·S Rottem, S Razin
Oct 1, 1974·Journal of the National Cancer Institute·R B HerbermanK S Chang
Dec 1, 1974·Infection and Immunity·C R RinaldoL A Glasgow
Jul 28, 1967·Annals of the New York Academy of Sciences·N L SomersonR M Chanock
Oct 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·S RazinR M Chanock
Jan 1, 1980·European Journal of Immunology·F Loor
Jan 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·R M CHANOCKM F BARILE

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Citations

Jul 15, 1982·Klinische Wochenschrift·H KirchnerE Storch
Sep 1, 1996·FEMS Immunology and Medical Microbiology·C S Zurita-SalinasJ Alcocer-Varela
Jan 1, 1982·Journal of Interferon Research·J BeckJ Wietzerbin
Nov 15, 1983·International Journal of Cancer. Journal International Du Cancer·A GrönbergG Klein
Jul 15, 1984·International Journal of Cancer. Journal International Du Cancer·R BergerH Kirchner
Mar 15, 1983·International Journal of Cancer. Journal International Du Cancer·I KawaseC S Henney
May 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L M Reid, D M Jefferson
Sep 1, 1984·Cellular Immunology·D A WeigentJ E Blalock

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