PMID: 9531381Apr 8, 1998Paper

Interferons antagonize gamma-ray-induced depression of natural immunity

International Journal of Radiation Oncology, Biology, Physics
M P FuggettaE Bonmassar

Abstract

The aim of this study was to determine the inhibitory effects of in vitro radiation on the number and function of natural killer (NK) cells and to investigate the capability of interferons (IFNs) to restore the activity of NK, depressed by gamma-rays. Mononuclear cells (MNC) were obtained from intact or in vitro irradiated (20 Gy) peripheral blood collected from healthy donors. Alternatively, MNC were irradiated (20 Gy) after separation from intact whole blood. The in vitro treatment of MNC with IFNs (alpha, beta, or gamma, 200 UI/ml) was performed at different times after or before radiation. The NK activity (4 h-51Cr release test), the percentage of CD16+/CD56+ cells and apoptosis (cytometric analysis), and binding (microscopic observation) were evaluated on Days 0, 1, 2, and 5 from gamma-ray exposure and IFNs treatment. The in vitro treatment of irradiated MNC with betaIFN after radiation completely reverses the inhibitory effects of gamma-rays on human NK activity. BetaIFN do not reduce the apoptosis induction by radiation and don't modify the number of CD16- or CD56-positive cells. The binding between irradiated effectors and tumor cells (K562) appears partially increased in betaIFN-treated MNC. The results of the present ...Continue Reading

References

Jan 1, 1992·Cytometry·Z DarzynkiewiczF Traganos
Aug 1, 1989·Rheumatic Diseases Clinics of North America·S M Helfgott
May 1, 1988·The Journal of Infectious Diseases·J I Herrero-HerreroJ Batlle-Forondona
May 1, 1987·Journal of Clinical Immunology·K McGinnesR Penny
Jan 1, 1980·Acta Radiologica. Oncology·H BlomgrenJ Wasserman
Jan 1, 1982·Immunological Reviews·G DoriaL Adorini
Oct 1, 1983·European Journal of Cancer & Clinical Oncology·S RotsteinB Johansson
Mar 1, 1981·The Journal of Experimental Medicine·M Ullberg, M Jondal
Jun 3, 1994·Journal of Immunological Methods·W G TelfordP J Fraker
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·E A ReapP L Cohen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Related Papers

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
P AllavenaM D'Incalci
Dose-response : a Publication of International Hormesis Society
Ali Shabestani MonfaredAkbar Nasir
International Archives of Allergy and Applied Immunology
S E Christmas, M Moore
© 2022 Meta ULC. All rights reserved