Intergenic regions in the rhoptry associated protein-1 (rap-1) locus promote exogenous gene expression in Babesia bovis

International Journal for Parasitology
Carlos E SuarezTerry F McElwain

Abstract

Members of the Babesiarap-1 gene family are expressed during multiple parasite stages, and are regulated by both transcriptional and post-transcriptional mechanisms. In all Babesia species, tandemly arranged rap-1 gene copies are separated by an intergenic (IG) region that is hypothesized to regulate gene expression. In this study, we tested that hypothesis by determining whether the Babesia bovisrap-1 IG region could promote extra-chromosomal expression of exogenous genes introduced into merozoites by transfection, and whether a tandem arrangement of IG regions similar to the rap-1 locus enhances exogenous gene expression. Initially, electroporation conditions of B. bovis parasites were determined using expression of the reporter luciferase gene. Both B. bovis transfected by electroporation and Escherichia coli transformed with plasmid p40-15-luc containing the luciferase gene under the control of the B. bovisrap-1 IG and 3' flanking regions were able to express luciferase, indicating that the rap-1 IG region contains a functional promoter. The chromosomal organization of the B. bovisrap-1 locus includes two identical rap-1 open reading frames and IG regions in a head to tail orientation. To determine whether this orientation ...Continue Reading

References

Jun 1, 1982·Parasitology·D A PalmerC A Carson
Feb 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y WuT E Wellems
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y WuT E Wellems
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·B S Crabb, A F Cowman
Mar 13, 1998·Molecular and Biochemical Parasitology·B S CrabbA F Cowman
Jul 15, 1998·Molecular and Biochemical Parasitology·C E SuarezT F McElwain
Jun 22, 1999·Molecular and Biochemical Parasitology·T F de Koning-WardC J Janse
Jun 23, 1999·Parasitology Today·W C Brown, G H Palmer
Oct 6, 2000·Annual Review of Microbiology·T F de Koning-WardA P Waters
Sep 12, 2001·Molecular and Biochemical Parasitology·M J Blackman, L H Bannister
Jul 27, 2002·International Journal of Medical Microbiology : IJMM·Jeremy GrayMarta Granström
Dec 19, 2002·Parasitology Research·Kevin T Militello, Dyann F Wirth
Apr 4, 2003·Molecular and Biochemical Parasitology·Carlos E SuarezTerry F McElwain

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Citations

Jul 6, 2010·Trends in Parasitology·Sejal GohilBrian M Cooke
Oct 4, 2008·International Journal for Parasitology·Carlos E Suarez, Terry F McElwain
Dec 14, 2007·Experimental Parasitology·Carlos E Suarez, Terry F McElwain
Mar 24, 2016·Parasites & Vectors·Hassan HakimiMasahito Asada
Aug 10, 2005·Veterinary Parasitology·Brian M CookeRoss L Coppel
Sep 25, 2012·International Journal for Parasitology·Joachim Müller, Andrew Hemphill
Jun 21, 2011·Veterinary Parasitology·Carlos E Suarez, Susan Noh
Oct 17, 2012·International Journal for Parasitology·Sejal GohilBrian M Cooke
Feb 1, 2015·Veterinary Parasitology·T MoladA Markovics
Oct 28, 2016·Parasites & Vectors·Guiquan GuanRobin B Gasser
Jul 30, 2014·Parasitology·Monica Florin-ChristensenLeonhard Schnittger
Jul 22, 2017·Molecular and Biochemical Parasitology·Mingming LiuXuenan Xuan
Sep 13, 2017·International Journal for Parasitology·C E SuarezB M Cooke

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